Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Membranes and Filtration
2.2. D Imaging and Analysis
3. Results and Discussion
3.1. Membrane Structure Imaging
3.2. Liposome Retention Imaging
3.3. Bacterial Challenges
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Membrane | Location | Permeability (µm2) | Tortuosity Factor |
---|---|---|---|
Upstream | Upper third | 0.0279 ± 0.0015 | 1.21 ± 0.02 |
Central third | 0.0257 ± 0.0013 | 1.24 ± 0.02 | |
Lower third | 0.0139 ± 0.0011 | 1.32 ± 0.05 | |
Downstream | Upper third | 0.0013 ± 0.0004 | 2.47 ± 0.58 |
Central third | 0.0013 ± 0.0004 | 2.47 ± 0.59 | |
Lower third | 0.0013 ± 0.0004 | 2.51 ± 0.61 |
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Johnson, T.F.; Jones, K.; Iacoviello, F.; Turner, S.; Jackson, N.B.; Zourna, K.; Welsh, J.H.; Shearing, P.R.; Hoare, M.; Bracewell, D.G. Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy. Membranes 2021, 11, 905. https://doi.org/10.3390/membranes11110905
Johnson TF, Jones K, Iacoviello F, Turner S, Jackson NB, Zourna K, Welsh JH, Shearing PR, Hoare M, Bracewell DG. Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy. Membranes. 2021; 11(11):905. https://doi.org/10.3390/membranes11110905
Chicago/Turabian StyleJohnson, Thomas F., Kyle Jones, Francesco Iacoviello, Stephen Turner, Nigel B. Jackson, Kalliopi Zourna, John H. Welsh, Paul R. Shearing, Mike Hoare, and Daniel G. Bracewell. 2021. "Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy" Membranes 11, no. 11: 905. https://doi.org/10.3390/membranes11110905