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Volume 9
Issue 3
10.3390/vaccines9030216
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Article

Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against Salmonella Enteritidis in Hens

by
Javier Ochoa-Repáraz
1,2,
Eduard Sebastià
3,
Marta Sitjà
3,
Ibai Tamayo
1,
Juan Manuel Irache
4 and
Carlos Gamazo
1,*
1
Department of Microbiology and Parasitology, Universidad de Navarra, 31008 Pamplona, Spain
2
Department of Biology, Eastern Washington University, Cheney, WA 99004, USA
3
Hipra Scientific, S.L., 17170 Amer, Spain
4
Department of Chemistry and Pharmaceutical Technology, Universidad de Navarra, 31008 Pamplona, Spain
*
Author to whom correspondence should be addressed.
Vaccines 2021, 9(3), 216; https://doi.org/10.3390/vaccines9030216
Submission received: 3 February 2021 / Revised: 23 February 2021 / Accepted: 23 February 2021 / Published: 3 March 2021
(This article belongs to the Special Issue Poultry Vaccines)
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Abstract

Salmonellosis remains a major medical and an unmet socioeconomic challenge. Worldwide, more than three million deaths per year are associated with Salmonella enterica serovar Enteritidis infections. Although commercially available vaccines for use in poultry exist, their efficacy is limited. We previously described a method for isolating a heat extract (HE) fraction of the cell surface of S. Enteritidis that contained major antigenic complexes immunogenic in hens naturally infected with the bacterium. One single dose of S. Enteritidis’ HE induced protection against lethal salmonellosis in mice. Furthermore, HE encapsulation in nanoparticles of the copolymer of methyl vinyl ether and maleic anhydride (PVM/MA), Gantrez AN, improved and prolonged the protection against the disease in mice. We formulated new preparations of Gantrez AN nanoparticles with HE S. Enteritidis and assessed their stability in drinking water and their efficacy in hens after experimental infection. The oral treatment of six-week-old hens with two doses of HE nanoparticles significantly reduced the Salmonella excretion in hens. Due to the effectiveness of the treatment in reducing bacterial excretion, we conclude that HE nanoencapsulation obtained from S. Enteritidis is a viable novel vaccination approach against salmonellosis in farms.
Keywords: Salmonella enteritidis; vaccine; nanoparticles; hens Salmonella enteritidis; vaccine; nanoparticles; hens

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MDPI and ACS Style

Ochoa-Repáraz, J.; Sebastià, E.; Sitjà, M.; Tamayo, I.; Irache, J.M.; Gamazo, C. Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against Salmonella Enteritidis in Hens. Vaccines 2021, 9, 216. https://doi.org/10.3390/vaccines9030216

AMA Style

Ochoa-Repáraz J, Sebastià E, Sitjà M, Tamayo I, Irache JM, Gamazo C. Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against Salmonella Enteritidis in Hens. Vaccines. 2021; 9(3):216. https://doi.org/10.3390/vaccines9030216

Chicago/Turabian Style

Ochoa-Repáraz, Javier, Eduard Sebastià, Marta Sitjà, Ibai Tamayo, Juan Manuel Irache, and Carlos Gamazo. 2021. "Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against Salmonella Enteritidis in Hens" Vaccines 9, no. 3: 216. https://doi.org/10.3390/vaccines9030216

APA Style

Ochoa-Repáraz, J., Sebastià, E., Sitjà, M., Tamayo, I., Irache, J. M., & Gamazo, C. (2021). Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against Salmonella Enteritidis in Hens. Vaccines, 9(3), 216. https://doi.org/10.3390/vaccines9030216

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