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A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization
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A GFP Reporter MR766-Based Flow Cytometry Neutralization Test for Rapid Detection of Zika Virus-Neutralizing Antibodies in Serum Specimens

Université de La Réunion, INSERM U1187, CNRS UMR 9192, IRD UMR 249,
Unité Mixte Processus Infectieux en Milieu Insulaire Tropical, Plateforme Technologique CYROI, 97491 Sainte-Clotilde, La Réunion, France
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Author to whom correspondence should be addressed.
Vaccines 2019, 7(3), 66; https://doi.org/10.3390/vaccines7030066
Received: 26 June 2019 / Revised: 12 July 2019 / Accepted: 16 July 2019 / Published: 16 July 2019
(This article belongs to the Special Issue Current Flavivirus Research Important for Vaccine Development )
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Abstract

Zika virus (ZIKV) is an emerging arthropod-borne virus of major public health concern. ZIKV infection is responsible for congenital Zika disease and other neurological defects. Antibody-mediated virus neutralization is an essential component of protective antiviral immunity against ZIKV. In the present study, we assessed whether our GFP reporter ZIKV derived from African viral strain MR766 could be useful for the development of a flow cytometry neutralization test (FNT), as an alternative to the conventional plaque-reduction neutralization test (PRNT). To improve the efficacy of GFP-expressing MR766, we selected virus variant MR766GFP showing a high level of GFP signal in infected cells. A MR766GFP-based FNT was assayed with immune sera from adult mice that received ZIKBeHMR-2. The chimeric ZIKV clone ZIKBeHMR-2 comprises the structural protein region of epidemic strain BeH819015 into MR766 backbone. We reported that adult mice inoculated with ZIKBeHMR-2 developed high levels of neutralizing anti-ZIKV antibodies. Comparative analysis between MR766GFP-based FNT and conventional PRNT was performed using mouse anti-ZIKBeHMR-2 immune sera. Indistinguishable neutralization patterns were observed when compared with PRNT50 and FNT50. We consider that the newly developed MR766GFP-based FNT is a valid format for measuring ZIKV-neutralizing antibodies in serum specimens. View Full-Text
Keywords: arbovirus; Zika virus; GFP reporter ZIKV; flow cytometry; neutralization test; mouse immune sera; humoral immune response; virus neutralizing antibodies; plaque-reduction neutralization test; flow cytometry neutralization test arbovirus; Zika virus; GFP reporter ZIKV; flow cytometry; neutralization test; mouse immune sera; humoral immune response; virus neutralizing antibodies; plaque-reduction neutralization test; flow cytometry neutralization test
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Frumence, E.; Viranaicken, W.; Gadea, G.; Desprès, P. A GFP Reporter MR766-Based Flow Cytometry Neutralization Test for Rapid Detection of Zika Virus-Neutralizing Antibodies in Serum Specimens. Vaccines 2019, 7, 66.

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