A GFP Reporter MR766-Based Flow Cytometry Neutralization Test for Rapid Detection of Zika Virus-Neutralizing Antibodies in Serum Specimens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Virus
2.2. Generation of MR766GFP
2.3. Focus-Forming Assay
2.4. Mouse Serum Specimens
2.5. Plaque-Reduction Neutralization Test
2.6. Flow Cytometry Assay and Flow Cytometry-Based Neutralization Test
2.7. Statistical Analysis
3. Results
3.1. Generation of Viral Clone MR766GFP for Flow Cytometry Neutralization Test
3.2. Comparison Between MR766GFP-Based FNT Assay and Conventional PRNT
4. Discussion
5. Conclusions
6. Patent
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Frumence, E.; Viranaicken, W.; Gadea, G.; Desprès, P. A GFP Reporter MR766-Based Flow Cytometry Neutralization Test for Rapid Detection of Zika Virus-Neutralizing Antibodies in Serum Specimens. Vaccines 2019, 7, 66. https://doi.org/10.3390/vaccines7030066
Frumence E, Viranaicken W, Gadea G, Desprès P. A GFP Reporter MR766-Based Flow Cytometry Neutralization Test for Rapid Detection of Zika Virus-Neutralizing Antibodies in Serum Specimens. Vaccines. 2019; 7(3):66. https://doi.org/10.3390/vaccines7030066
Chicago/Turabian StyleFrumence, Etienne, Wildriss Viranaicken, Gilles Gadea, and Philippe Desprès. 2019. "A GFP Reporter MR766-Based Flow Cytometry Neutralization Test for Rapid Detection of Zika Virus-Neutralizing Antibodies in Serum Specimens" Vaccines 7, no. 3: 66. https://doi.org/10.3390/vaccines7030066