A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells, Viruses, and Reagents
2.2. Recovering of Viral Clone ZIKBeHMR-2
2.3. Cytotoxicity Assay
2.4. Immunodetection of Viral Proteins
2.5. Mouse Experiment and Ethical Statement
2.6. Preparation of ZIKBeHMR-2 Samples for Mouse Experiments
2.7. Indirect ELISA
2.8. Seroneutralization Assay
2.9. Statistical Analysis
3. Results
3.1. Characterization of Viral Clone ZIKBeHMR-2
3.2. ZIKVBeHMR-2 Elicits anti-ZIKV Antibody Response in BALB/c Mice
3.3. ZIKBeHMR-2 Stimulates a Rapid Production of Neutralizing Anti-ZIKV Antibodies
3.4. Neutralizing Activity of Anti-ZIKBeHMR-2 Antibodies against Various ZIKV Strains
4. Discussion
5. Conclusions
6. Patent
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Immune Sera | |||
---|---|---|---|
Controls 1 | Weeks | Anti-ZIKV.rEDIII 2 | PRNT50 3 |
PBS/FBS | 4 | <50 | <50 |
Cell supernatant | 4 | <50 | <50 |
Inactivated ZIKV | 4 | <50 | <50 |
ZIKBeHMR-2 4 | |||
1st dose | 4 | 1083 (844–1391) | 529 (385–726) |
2nd dose | 10 | 6174 (3733–10212) | 6032 (4039–9009) |
Viral Strain | PRNT50 1 | |
---|---|---|
1st Dose | 2nd Dose | |
ZIKBeHMR-1 | ≤50 | 183 (73–457) |
ZIKBeHMR-2 | 465 (274–789) | 5824 (4320–7851) |
BR15MC | ≤50 | 296 (159–550) |
MR766MC | 245 (124–483) | 3480 (1472–8229) |
PF-25013-18 | ≤50 | 170 (97–296) |
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Frumence, E.; Viranaicken, W.; Bos, S.; Alvarez-Martinez, M.-T.; Roche, M.; Arnaud, J.-D.; Gadea, G.; Desprès, P. A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization. Vaccines 2019, 7, 55. https://doi.org/10.3390/vaccines7020055
Frumence E, Viranaicken W, Bos S, Alvarez-Martinez M-T, Roche M, Arnaud J-D, Gadea G, Desprès P. A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization. Vaccines. 2019; 7(2):55. https://doi.org/10.3390/vaccines7020055
Chicago/Turabian StyleFrumence, Etienne, Wildriss Viranaicken, Sandra Bos, Maria-Teresa Alvarez-Martinez, Marjolaine Roche, Jacques-Damien Arnaud, Gilles Gadea, and Philippe Desprès. 2019. "A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization" Vaccines 7, no. 2: 55. https://doi.org/10.3390/vaccines7020055