Review Reports

Round 1

Reviewer 1 Report

The focuses of the manuscript were on the local response and barrier recovery after applying high-density microarray patches (HD-MP) onto human skin. The participants are adults aged greater than 65 year old. In the previous studies, they were adults aged between 18-64. Similar results were found in both studies. However, more tests should be done as previous studies have demonstrated that mechanical stress, induce inflammatory response and cell death were observed.

Author Response

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Reviewer 2 Report

Although the paper is well presented and well writen, I have some concerns with the evaluation of the presence of inflammatory markers; the author do confess in their discussion that this later was not addressed in the current study. I woud suggest to address this  point and compare them with inflammatory responses of the young skin.

 

Author Response

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Reviewer 3 Report

In this paper Iredahl et al. have reported local response and barrier recovery in elderly skin after application of high density microneedle patches.

The study is well designed and adequate experiments are condicuted to support the conclusions. The findings are of interest and would add to our understanding of effect of microneedle usage in elderly subjects

I have no specific comments and recommend acceptance

Author Response

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Round 2

Reviewer 1 Report

The revised manuscript is similar to the first version.  A revised vresion should have descriptions on the following issues:

1)  the comparison between the invasive and non-invasive methods, on the physiological change of skin (in the Introduction section),

2) the reason for choosing the non-invasive method (in the method section),

3)  the studies on the young skin should be stated as the future work.

Author Response

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Reviewer 2 Report

Detail points raised in my previous review have been adressed adequatly.

Author Response

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Author Response File: Author Response.pdf