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Volume 10
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10.3390/vaccines10020226
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Article

Efficacy of Multivalent, Cochleate-Based Vaccine against Salmonella Infantis, S. Enteritidis and S. Typhimurium in Laying Hens

by
Leonardo Sáenz
1,
Miguel Guzmán
1,2,
Sonia Vidal
1,
Mario Caruffo
1,3,
Daniela Siel
1,
Caridad Zayas
1,
Rodolfo Paredes
4,
Carolina Valenzuela
1,
Héctor Hidalgo
1,
Oliver Pérez
5 and
Lisette Lapierre
1,6,*
1
Faculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, Chile
2
Núcleo de Investigaciones Aplicadas en Ciencias Veterinarias y Agronómicas, NIAVA, Facultad de Medicina Veterinaria y Agronomía, Campus Maipú–Sede Santiago, Universidad de las Américas, Santiago 9251454, Chile
3
Escuela de Biotecnología, Facultad de Ciencias, Universidad Santo Tomás, Santiago 8370003, Chile
4
Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370035, Chile
5
Instituto de Ciencias Básicas Y Preclínicas “Victoria de Girón”, Universidad de Ciencias Médicas de La Habana, Havana 10600, Cuba
6
Lisette Lapierre, Faculty of Veterinary Sciences, University of Chile, Santiago 8820808, Chile
*
Author to whom correspondence should be addressed.
Vaccines 2022, 10(2), 226; https://doi.org/10.3390/vaccines10020226
Submission received: 8 October 2021 / Revised: 30 December 2021 / Accepted: 12 January 2022 / Published: 1 February 2022
(This article belongs to the Special Issue Poultry Vaccines)
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Abstract

Salmonella enterica is an important foodborne pathogen. Commercial poultry are the main reservoirs of Salmonella enterica, leading to the contamination of food and outbreaks in humans. The vaccination of chickens is one of the most important strategies to reduce the number of Salmonella in poultry farms. Unfortunately, commercial vaccines have not been fully effective in controlling the spread and do not contain all the Salmonella serovars that circulate on farms. In this study, we evaluate a new, cochleate-based, trivalent injectable vaccine against S. Enteritidis, S. Typhimurium and S. Infantis, describing the vaccine security, capacity to induce specific anti-Salmonella serovar IgY and the gene expression of immune markers related to CD4 and CD8 T-cell-mediated immunity. Efficacy was evaluated through oral challenges performed separately for each Salmonella serotype. The efficacy and safety of the trivalent vaccine was proven under controlled conditions. The vaccine has no local or systemic reactions or adverse effects on poultry performance related to the vaccine. The vaccine provided significantly increased serum IgY titer levels, significantly reduced Salmonella CFU/g present in the cecum and an increased CD4+/CD8+ ratio in vaccinated animals when challenged with S. Infantis, S. Enteritidis and S. Typhimurium. These results indicate that this new trivalent vaccine does not generate adverse effects in poultry and produces an increase in neutralizing antibodies against the three Salmonella serovars.
Keywords: Salmonella Infantis; vaccine; cochleate; ELISA; poultry Salmonella Infantis; vaccine; cochleate; ELISA; poultry

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MDPI and ACS Style

Sáenz, L.; Guzmán, M.; Vidal, S.; Caruffo, M.; Siel, D.; Zayas, C.; Paredes, R.; Valenzuela, C.; Hidalgo, H.; Pérez, O.; et al. Efficacy of Multivalent, Cochleate-Based Vaccine against Salmonella Infantis, S. Enteritidis and S. Typhimurium in Laying Hens. Vaccines 2022, 10, 226. https://doi.org/10.3390/vaccines10020226

AMA Style

Sáenz L, Guzmán M, Vidal S, Caruffo M, Siel D, Zayas C, Paredes R, Valenzuela C, Hidalgo H, Pérez O, et al. Efficacy of Multivalent, Cochleate-Based Vaccine against Salmonella Infantis, S. Enteritidis and S. Typhimurium in Laying Hens. Vaccines. 2022; 10(2):226. https://doi.org/10.3390/vaccines10020226

Chicago/Turabian Style

Sáenz, Leonardo, Miguel Guzmán, Sonia Vidal, Mario Caruffo, Daniela Siel, Caridad Zayas, Rodolfo Paredes, Carolina Valenzuela, Héctor Hidalgo, Oliver Pérez, and et al. 2022. "Efficacy of Multivalent, Cochleate-Based Vaccine against Salmonella Infantis, S. Enteritidis and S. Typhimurium in Laying Hens" Vaccines 10, no. 2: 226. https://doi.org/10.3390/vaccines10020226

APA Style

Sáenz, L., Guzmán, M., Vidal, S., Caruffo, M., Siel, D., Zayas, C., Paredes, R., Valenzuela, C., Hidalgo, H., Pérez, O., & Lapierre, L. (2022). Efficacy of Multivalent, Cochleate-Based Vaccine against Salmonella Infantis, S. Enteritidis and S. Typhimurium in Laying Hens. Vaccines, 10(2), 226. https://doi.org/10.3390/vaccines10020226

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