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Article

Effect of Heme Oxygenase-1 on Melanoma Development in Mice—Role of Tumor-Infiltrating Immune Cells

1
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland
2
Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine, 04-141 Warsaw, Poland
3
Center for Translational Research and Molecular Biology of Cancer, Maria Skłodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, Poland
4
Department of Pathology, Oncology Center, 31-115 Krakow, Poland
5
Department of Ergonomics and Exercise Physiology, Faculty of Health Science, Jagiellonian University Medical College, 31-126 Krakow, Poland
6
Transplantation Centre, Jagiellonian University, 30-663 Krakow, Poland
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(12), 1223; https://doi.org/10.3390/antiox9121223
Received: 29 October 2020 / Revised: 25 November 2020 / Accepted: 1 December 2020 / Published: 3 December 2020
(This article belongs to the Special Issue Pharmacological and Clinical Significance of Heme Oxygenase-1)
Objective: Heme oxygenase-1 (HO-1) is a cytoprotective, proangiogenic and anti-inflammatory enzyme that is often upregulated in tumors. Overexpression of HO-1 in melanoma cells leads to enhanced tumor growth, augmented angiogenesis and resistance to anticancer treatment. The effect of HO-1 in host cells on tumor development is, however, hardly known. Methods and results: To clarify the effect of HO-1 expression in host cells on melanoma progression, C57BL/6xFvB mice of different HO-1 genotypes, HO-1+/+, HO-1+/−, and HO-1−/−, were injected with the syngeneic wild-type murine melanoma B16(F10) cell line. Lack of HO-1 in host cells did not significantly influence the host survival. Nevertheless, in comparison to the wild-type counterparts, the HO-1+/− and HO-1−/− males formed bigger tumors, and more numerous lung nodules; in addition, more of them had liver and spleen micrometastases. Females of all genotypes developed at least 10 times smaller tumors than males. Of importance, the growth of primary and secondary tumors was completely blocked in HO-1+/+ females. This was related to the increased infiltration of leukocytes (mainly lymphocytes T) in primary tumors. Conclusions: Although HO-1 overexpression in melanoma cells can enhance tumor progression in mice, its presence in host cells, including immune cells, can reduce growth and metastasis of melanoma. View Full-Text
Keywords: cancer; melanoma; heme oxgenase-1 (HO-1); female; tumor-infiltrating immune cells cancer; melanoma; heme oxgenase-1 (HO-1); female; tumor-infiltrating immune cells
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MDPI and ACS Style

Was, H.; Cichon, T.; Smolarczyk, R.; Lackowska, B.; Mazur-Bialy, A.; Mazur, M.; Szade, A.; Dominik, P.; Mazan, M.; Kotlinowski, J.; Zebzda, A.; Kusienicka, A.; Kieda, C.; Dulak, J.; Jozkowicz, A. Effect of Heme Oxygenase-1 on Melanoma Development in Mice—Role of Tumor-Infiltrating Immune Cells. Antioxidants 2020, 9, 1223. https://doi.org/10.3390/antiox9121223

AMA Style

Was H, Cichon T, Smolarczyk R, Lackowska B, Mazur-Bialy A, Mazur M, Szade A, Dominik P, Mazan M, Kotlinowski J, Zebzda A, Kusienicka A, Kieda C, Dulak J, Jozkowicz A. Effect of Heme Oxygenase-1 on Melanoma Development in Mice—Role of Tumor-Infiltrating Immune Cells. Antioxidants. 2020; 9(12):1223. https://doi.org/10.3390/antiox9121223

Chicago/Turabian Style

Was, Halina, Tomasz Cichon, Ryszard Smolarczyk, Bozena Lackowska, Agnieszka Mazur-Bialy, Magdalena Mazur, Agata Szade, Pawel Dominik, Milena Mazan, Jerzy Kotlinowski, Anna Zebzda, Anna Kusienicka, Claudine Kieda, Jozef Dulak, and Alicja Jozkowicz. 2020. "Effect of Heme Oxygenase-1 on Melanoma Development in Mice—Role of Tumor-Infiltrating Immune Cells" Antioxidants 9, no. 12: 1223. https://doi.org/10.3390/antiox9121223

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