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Open AccessArticle

Sangiovese cv Pomace Seeds Extract-Fortified Kefir Exerts Anti-Inflammatory Activity in an In Vitro Model of Intestinal Epithelium Using Caco-2 Cells

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Dipartimento di Farmacia e Scienze della Salute e della Nutrizione—Dipartimento di Eccellenza 2018-2022, Università della Calabria, Edificio Polifunzionale, 87036 Rende (CS), Italy
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Dipartimento di Biotecnologie, Chimica e Farmacia—Dipartimento di Eccellenza 2018-2022, Università di Siena, Via Aldo Moro 2, 53100 Siena, Italy
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Dipartimento di Scienze Fisiche, della Terra e dell’Ambiente, Università di Siena, Via Laterina 8, 53100 Siena, Italy
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Dipartimento di Chimica, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Roma, Italy
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(1), 54; https://doi.org/10.3390/antiox9010054
Received: 5 December 2019 / Revised: 4 January 2020 / Accepted: 5 January 2020 / Published: 8 January 2020
Inflammatory bowel disease and food allergies are a growing topic in the field of nutrition science. Polyphenols, which are the most important secondary metabolites of plants, demonstrated to modulate the expression and/or production of numerous proteins, but also to regulate the intestinal ecosystem. In this context, our aim was the investigation of protective effects against the gastrointestinal mucosa of fortified milk kefir obtained by adding seeds extract from Sangiovese cv. Pomace. Methods: An ultrasound-assisted method was used to obtain the extracts. All the extracts were assayed for the antioxidant activity. The best extract was used as an additive of fermented milk kefir to obtain a fortified final product. Kefir samples were analyzed by NMR spectroscopy. The efficiency of the barrier functions was evaluated by measuring trans-epithelial electric resistance (TEER) using a voltmeter. Results: the enriched kefir (Ksgn) possesses higher antioxidant performances compared to the unfortified sample (Kwht). Kwht and Ksgn did not alter Caco-2 TEER in basal condition. View Full-Text
Keywords: wine pomace; antioxidant activity; TEER; Caco-2 cell line; milk kefir; phenol red; polyphenols; extraction wine pomace; antioxidant activity; TEER; Caco-2 cell line; milk kefir; phenol red; polyphenols; extraction
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Carullo, G.; Governa, P.; Spizzirri, U.G.; Biagi, M.; Sciubba, F.; Giorgi, G.; Loizzo, M.R.; Di Cocco, M.E.; Aiello, F.; Restuccia, D. Sangiovese cv Pomace Seeds Extract-Fortified Kefir Exerts Anti-Inflammatory Activity in an In Vitro Model of Intestinal Epithelium Using Caco-2 Cells. Antioxidants 2020, 9, 54.

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