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Open AccessArticle

Isolation of Strong Antioxidants from Paeonia Officinalis Roots and Leaves and Evaluation of Their Bioactivities

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Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų˛ pl. 19, LT-50254 Kaunas, Lithuania
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IBET—Instituto de Biologia Experimental e Tecnológica, Food & Health Division Apartado 12, 2780-901 Oeiras, Portugal
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(8), 249; https://doi.org/10.3390/antiox8080249
Received: 27 June 2019 / Revised: 17 July 2019 / Accepted: 25 July 2019 / Published: 27 July 2019
(This article belongs to the Special Issue Antioxidant Activity of Polyphenolic Plant Extracts)
Paeonia officinalis extracts from leaves and roots were tested for their antioxidant potential using in vitro chemical (Folin-Ciocalteu, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), oxygen radical absorbance capacity (ORAC), hydroxyl radical antioxidant capacity (HORAC), hydroxyl radical scavenging capacity HOSC)) and cellular antioxidant activity (CAA) assays. Leaf extracts were stronger antioxidants than root extracts, while methanol was a more effective solvent than water in chemical assays. However, the selected water extract of leaves was a stronger antioxidant in CAA than the methanol extract (0.106 vs. 0.046 µmol quercetin equivalents/mg). Twenty compounds were identified by ultra performance liquid chromatography-quadrupole-time-of-flight (UPLC-Q-TOF) mass spectrometer, while on-line screening of their antioxidant capacity by high performance liquid chromatography (HPLC) with a DPPH-scavenging detector revealed that gallic acid derivatives are the major peony antioxidants. Root water and leaf methanol extracts inhibited α-amylase in a dose dependent manner. The IC50 value for the strongest inhibitor, the methanol extract of leaves, was 1.67 mg/mL. In addition, the cytotoxicity assessment of extracts using human Caco-2 cells demonstrated that none of them possessed cytotoxic effects. View Full-Text
Keywords: P. officinalis; phytochemicals; antioxidant capacity; cytotoxicity; α-amylase inhibition P. officinalis; phytochemicals; antioxidant capacity; cytotoxicity; α-amylase inhibition
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Dienaitė, L.; Pukalskienė, M.; Pukalskas, A.; Pereira, C.V.; Matias, A.A.; Venskutonis, P.R. Isolation of Strong Antioxidants from Paeonia Officinalis Roots and Leaves and Evaluation of Their Bioactivities. Antioxidants 2019, 8, 249.

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