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The Effect of Hydrogen Sulfide on Different Parameters of Human Plasma in the Presence or Absence of Exogenous Reactive Oxygen Species

Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/3, 90-236 Lodz, Poland
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Antioxidants 2019, 8(12), 610; https://doi.org/10.3390/antiox8120610
Received: 12 November 2019 / Revised: 28 November 2019 / Accepted: 29 November 2019 / Published: 3 December 2019
The main aim of the study is to examine the effect of sodium hydrosulfide (NaHS), an H2S donor, on the oxidative stress in human plasma in vitro. It also examined the effects of very high concentrations of exogenous hydrogen sulfide on the hemostatic parameters (coagulation and fibrinolytic activity) of human plasma. Plasma was incubated for 5–30 min with different concentrations of NaHS from 0.01 to 10 mM. Following this, lipid peroxidation was measured as a thiobarbituric acid reactive substance (TBARS) concentration and the oxidation of amino acid residues in proteins was measured by determining the amounts of thiol groups and carbonyl groups. Hydrogen peroxide (H2O2) and the hydroxyl radical generating oxidation system (Fe/H2O2) were used as oxidative stress inducers. Hemostatic factors, such as the maximum velocity of clot formation, fibrin lysis half-time, the activated partial thromboplastin time (APTT), thrombin time (TT), and international normalized ratio (INR), were estimated. Changes in lipid peroxidation, carbonyl group formation, and thiol group oxidation were detected at high concentrations of H2S (0.1–10 mM), and these results indicate that NaHS (as the precursor of H2S) may have pro-oxidative effects in human plasma in vitro. Moreover, considering the data presented in this study, we suggest that the oxidative stress stimulated by NaHS (at high concentrations: 1–10 mM) is not involved in changes of the hemostatic activity of plasma. View Full-Text
Keywords: hydrogen sulfide; plasma; oxidative stress; pro-oxidant; hemostasis hydrogen sulfide; plasma; oxidative stress; pro-oxidant; hemostasis
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Olas, B.; Brodek, P.; Kontek, B. The Effect of Hydrogen Sulfide on Different Parameters of Human Plasma in the Presence or Absence of Exogenous Reactive Oxygen Species. Antioxidants 2019, 8, 610.

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