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Open AccessArticle

Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction

1
Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine
2
Faculty of Biology, Drohobych Ivan Franko State Pedagogical University, 82100 Drohobych, Ukraine
3
Institute of Analytical Sciences, UMR 5280, CNRS, Université Claude Bernard Lyon 1, ENS Lyon-5, 5 rue de la Doua, F-69100 Villeurbanne, France
4
Department of Chemical Engineering, Biotechnology and Materials, Ariel University, Ariel 4070000, Israel
*
Authors to whom correspondence should be addressed.
Appl. Sci. 2019, 9(4), 720; https://doi.org/10.3390/app9040720
Received: 30 December 2018 / Revised: 13 February 2019 / Accepted: 14 February 2019 / Published: 19 February 2019
(This article belongs to the Special Issue Nano-Biointerface for Biosensing)
Novel nanomaterials, including metallic nanoparticles obtained via green synthesis (gNPs), have a great potential for application in biotechnology, industry and medicine. The special role of gNPs is related to antibacterial agents, fluorescent markers and carriers for drug delivery. However, application of gNPs for construction of amperometric biosensors (ABSs) is not well documented. The aim of the current research was to study potential advantages of using gNPs in biosensorics. The extracellular metabolites of the yeast Ogataea polymorpha were used as reducing agents for obtaining gNPs from the corresponding inorganic ions. Several gNPs were synthesized, characterized and tested as enzyme carriers on the surface of graphite electrodes (GEs). The most effective were Pd-based gNPs (gPdNPs), and these were studied further and applied for construction of laccase- and alcohol oxidase (AO)-based ABSs. AO/GE, AO-gPdNPs/GE, laccase/GE and laccase-gPdNPs/GE were obtained, and their analytical characteristics were studied. Both gPdNPs-modified ABSs were found to have broader linear ranges and higher storage stabilities than control electrodes, although they are less sensitive toward corresponding substrates. We thus conclude that gPdNPs may be promising for construction of ABSs for enzymes with very high affinities to their substrates. View Full-Text
Keywords: green synthesis; metallic nanoparticles; extracellular metabolites; yeast Ogataea polymorpha; laccase; alcohol oxidase; amperometric biosensors green synthesis; metallic nanoparticles; extracellular metabolites; yeast Ogataea polymorpha; laccase; alcohol oxidase; amperometric biosensors
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MDPI and ACS Style

Gayda, G.Z.; Demkiv, O.M.; Stasyuk, N.Y.; Serkiz, R.Y.; Lootsik, M.D.; Errachid, A.; Gonchar, M.V.; Nisnevitch, M. Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction. Appl. Sci. 2019, 9, 720. https://doi.org/10.3390/app9040720

AMA Style

Gayda GZ, Demkiv OM, Stasyuk NY, Serkiz RY, Lootsik MD, Errachid A, Gonchar MV, Nisnevitch M. Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction. Applied Sciences. 2019; 9(4):720. https://doi.org/10.3390/app9040720

Chicago/Turabian Style

Gayda, Galina Z.; Demkiv, Olha M.; Stasyuk, Nataliya Y.; Serkiz, Roman Y.; Lootsik, Maksym D.; Errachid, Abdelhamid; Gonchar, Mykhailo V.; Nisnevitch, Marina. 2019. "Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction" Appl. Sci. 9, no. 4: 720. https://doi.org/10.3390/app9040720

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