Next Article in Journal
Step-Pulse Modulation of Gain-Switched Semiconductor Pulsed Laser
Previous Article in Journal
Numerical Study on Dynamic-Stall Characteristics of Finite Wing and Rotor
Article Menu

Export Article

Open AccessArticle
Appl. Sci. 2019, 9(3), 601; https://doi.org/10.3390/app9030601

In Vitro Probiotic Potential of Lactic Acid Bacteria Isolated from Aguamiel and Pulque and Antibacterial Activity Against Pathogens

1
Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Circuito Ex Hacienda La Concepción S/N, Carretera Pachuca-Actopan, San Agustín Tlaxiaca 42160, Hidalgo, Mexico
2
Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo. Av. Universidad Km 1, Rancho Universitario, Tulancingo 43600, Hidalgo, Mexico
3
Laboratorio de Infectología, Departamento de Infectología, Hospital Infantil de México Federico Gómez, Col. Doctores, Delegación: Cuauhtémoc, Ciudad de México 06720, Mexico
*
Author to whom correspondence should be addressed.
Received: 18 January 2019 / Revised: 1 February 2019 / Accepted: 8 February 2019 / Published: 12 February 2019
(This article belongs to the Section Applied Biosciences and Bioengineering)
Full-Text   |   PDF [641 KB, uploaded 13 February 2019]   |  

Abstract

Probiotics can act as a natural barrier against several pathogens, such Helicobacter pylori, a bacterium linked to stomach cancer. The aim of the present study was to isolate and identify lactic acid bacteria (LAB) from pulque and aguamiel, and evaluate their probiotic potential and antimicrobial effect on Escherichia coli, Staphylococcus aureus, and Helicobacter pylori. Ten isolates were selected and evaluated for in vitro resistance to antibiotics and gastrointestinal conditions, and antimicrobial activity against E. coli and S. aureus and the effect on H. pylori strains. 16S rRNA identification was performed. Ten potential probiotic isolates were confirmed as belonging to the genera Lactobacillus and Pediococcus. All the strains were susceptible to clinical antibiotics, except to vancomycin. Sixty percent of the isolates exhibited antimicrobial activity against E. coli and S. aureus. The growth of H. pylori ATCC 43504 was suppressed by all the LAB, and the urease activity from all the H. pylori strains was inhibited, which may decrease its chances for survival in the stomach. The results suggest that LAB isolated from pulque and aguamiel could be an option to establish a harmless relationship between the host and H. pylori, helping in their eradication therapy. View Full-Text
Keywords: antimicrobial activity; gastrointestinal conditions; Helicobacter pylori; Lactobacillus; Pediococcus; urease antimicrobial activity; gastrointestinal conditions; Helicobacter pylori; Lactobacillus; Pediococcus; urease
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Cervantes-Elizarrarás, A.; Cruz-Cansino, N.S.; Ramírez-Moreno, E.; Vega-Sánchez, V.; Velázquez-Guadarrama, N.; Zafra-Rojas, Q.Y.; Piloni-Martini, J. In Vitro Probiotic Potential of Lactic Acid Bacteria Isolated from Aguamiel and Pulque and Antibacterial Activity Against Pathogens. Appl. Sci. 2019, 9, 601.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Appl. Sci. EISSN 2076-3417 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top