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Open AccessArticle

Architecture and Viability of the Biofilms Formed by Nine Listeria Strains on Various Hydrophobic and Hydrophilic Materials

1
Department of Food Hygiene and Technology, Veterinary Faculty, University of León, 24004 León, Spain
2
Institute of Food Science and Technology, University of León, 24004 León, Spain
*
Author to whom correspondence should be addressed.
Appl. Sci. 2019, 9(23), 5256; https://doi.org/10.3390/app9235256
Received: 15 September 2019 / Revised: 7 November 2019 / Accepted: 13 November 2019 / Published: 3 December 2019
(This article belongs to the Special Issue Biofilms in Focus: A Threat to Foods)
Biofilms are a key factor in the persistence of Listeria in food processing plants, representing a potential source of foodstuff contamination. Nine Listeria strains (eight Listeria monocytogenes and one Listeria ivanovii) were studied by confocal laser scanning microscopy (CLSM) for their ability to form biofilm on glass, polystyrene, graphene and resin after 120 h of incubation at 12 °C. The relationship between cell surface hydrophobicity and biofilm formation was also investigated. On comparing the data for all the strains, similar (P > 0.05) biovolume values were obtained on glass (average 3.39 ± 1.69 µm3/µm2) and graphene (2.93 ± 1.14 µm3/µm2), while higher (P < 0.05) values were observed for polystyrene (4.39 ± 4.14 µm3/µm2). The highest (P < 0.01) biovolume levels were found in the biofilms formed on resin (7.35 ± 1.45 µm3/µm2), which also had the smallest biomass of inactivated cells (0.38 ± 0.37 µm3/µm2 vs. 1.20 ± 1.12 µm3/µm2 on the remaining surfaces; P < 0.001). No relationship was noted between cell surface hydrophobicity and biofilm-forming ability. View Full-Text
Keywords: Listeria monocytogenes; Listeria ivanovii; biofilm; cell surface hydrophobicity; glass; polystyrene; graphene; resin Listeria monocytogenes; Listeria ivanovii; biofilm; cell surface hydrophobicity; glass; polystyrene; graphene; resin
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MDPI and ACS Style

Rodríguez-Melcón, C.; Alonso-Calleja, C.; Capita, R. Architecture and Viability of the Biofilms Formed by Nine Listeria Strains on Various Hydrophobic and Hydrophilic Materials. Appl. Sci. 2019, 9, 5256.

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