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Article

Molecular Specific and Sensitive Detection of Pyrazinamide and Its Metabolite Pyrazinoic Acid by Means of Surface Enhanced Raman Spectroscopy Employing In Situ Prepared Colloids

by 1,2,3,†, 1,2,3,†, 1, 1,‡, 1,2,3, 4, 4, 1,2,3,* and 1,2,3
1
Leibniz Institute of Photonic Technology (IPHT)—Member of the Research Alliance “Leibniz Health Technologies”, Albert-Einstein-Straße 9, 07745 Jena, Germany
2
Friedrich-Schiller-University Jena, Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07743 Jena, Germany
3
InfectoGnostics Research Campus Jena, Centre for Applied Research, Philosophenweg 7, 07743 Jena, Germany
4
Laboratorio de Bioinformática y Biología Molecular, Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima 31, Peru
*
Author to whom correspondence should be addressed.
These Authors contributed equally.
Current address: CiS Forschungsinstitut für Mikrosensorik GmbH, Konrad-Zuse-Straße 14, 99099 Erfurt, Germany.
Appl. Sci. 2019, 9(12), 2511; https://doi.org/10.3390/app9122511
Received: 15 February 2019 / Revised: 28 May 2019 / Accepted: 29 May 2019 / Published: 20 June 2019
(This article belongs to the Special Issue Surfaced Enhanced Raman Scattering (SERS) in Disease Diagnosis)
The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 µm for PZA and 21 µm for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 μm to 50 μm for PZA and from 50 μm to 80 μm for POA. View Full-Text
Keywords: surface-enhanced Raman spectroscopy (SERS); personal medicine; drug resistant mycobacteria; quantitative SERS detection of PZA and POA; lab-on-a-Chip (LoC) surface-enhanced Raman spectroscopy (SERS); personal medicine; drug resistant mycobacteria; quantitative SERS detection of PZA and POA; lab-on-a-Chip (LoC)
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MDPI and ACS Style

Muehlig, A.; Jahn, I.J.; Heidler, J.; Jahn, M.; Weber, K.; Sheen, P.; Zimic, M.; Cialla-May, D.; Popp, J. Molecular Specific and Sensitive Detection of Pyrazinamide and Its Metabolite Pyrazinoic Acid by Means of Surface Enhanced Raman Spectroscopy Employing In Situ Prepared Colloids. Appl. Sci. 2019, 9, 2511. https://doi.org/10.3390/app9122511

AMA Style

Muehlig A, Jahn IJ, Heidler J, Jahn M, Weber K, Sheen P, Zimic M, Cialla-May D, Popp J. Molecular Specific and Sensitive Detection of Pyrazinamide and Its Metabolite Pyrazinoic Acid by Means of Surface Enhanced Raman Spectroscopy Employing In Situ Prepared Colloids. Applied Sciences. 2019; 9(12):2511. https://doi.org/10.3390/app9122511

Chicago/Turabian Style

Muehlig, Anna, Izabella J. Jahn, Jan Heidler, Martin Jahn, Karina Weber, Patricia Sheen, Mirko Zimic, Dana Cialla-May, and Juergen Popp. 2019. "Molecular Specific and Sensitive Detection of Pyrazinamide and Its Metabolite Pyrazinoic Acid by Means of Surface Enhanced Raman Spectroscopy Employing In Situ Prepared Colloids" Applied Sciences 9, no. 12: 2511. https://doi.org/10.3390/app9122511

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