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Keywords = quantitative SERS detection of PZA and POA

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13 pages, 4968 KB  
Article
Molecular Specific and Sensitive Detection of Pyrazinamide and Its Metabolite Pyrazinoic Acid by Means of Surface Enhanced Raman Spectroscopy Employing In Situ Prepared Colloids
by Anna Muehlig, Izabella J. Jahn, Jan Heidler, Martin Jahn, Karina Weber, Patricia Sheen, Mirko Zimic, Dana Cialla-May and Juergen Popp
Appl. Sci. 2019, 9(12), 2511; https://doi.org/10.3390/app9122511 - 20 Jun 2019
Cited by 7 | Viewed by 5815
Abstract
The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the [...] Read more.
The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 µm for PZA and 21 µm for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 μm to 50 μm for PZA and from 50 μm to 80 μm for POA. Full article
(This article belongs to the Special Issue Surfaced Enhanced Raman Scattering (SERS) in Disease Diagnosis)
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