1. Introduction
Early diagnosis is crucial to prevent disease progressions [
1]. The correlation between the mechanical characteristics of a soft tissue and its status has been highlighted by several studies in many cell types like cancer cells, epithelial cells and laminopathies associated with diseases of the nuclear membrane [
2]. In cancer cells, for example, the malignant transformation influences the mechanical properties by disruption and/or reorganization of cytoskeleton [
3]. The stiffness, the elastic modulus, and the dynamic viscosity are among the most commonly used mechanical quantities to evaluate the state of a soft tissue. However the possibility to identify a diseased soft tissue in situ, at the microscale, can increase significantly the possibility of the cure.
Different techniques are used to perform such measurements as micropipette aspiration [
4], magnetic bead twisting [
5], atomic force microscopy [
6], optical tweezers [
7] or mechanical tweezers [
8]. In recent years, researchers focused on the developing of micro-electro-mechanical systems (MEMS) tweezers because of their efficiency and relative simplicity with respect to other systems [
9]. New gripping tweezers have been built taking cue from the kinematics of articulated mechanisms [
10] as the property of parallelograms [
11,
12,
13,
14,
15]. More complex motion, with an increasing of the number of degrees of freedom, can be obtained adopting the concept of lumped compliance. In fact, concentrated flexibility has been used in flexure hinges in several investigations [
16,
17,
18,
19,
20]. In order to improve the displacement accuracy and to lower the stress levels in the flexible elements a new conjugate surfaces flexure hinge (CSFH) flexure has been proposed [
21,
22,
23,
24,
25] and optimized [
26,
27]. The system has shown promising results in terms of of versatility and applicability to different types of tissue [
28]. Thanks to their high level of miniaturization, MEMS-Technology based tweezers can be employed also in minimal invasive [
29,
30,
31] or gastrointestinal surgery [
32], and more generally speaking, in endoluminal surgery, for example, in TEM [
33,
34,
35].
The actuation of the tweezer can be obtained by different systems, such as linear electrostatic actuators [
36,
37], rotatory electrostatic actuators [
38,
39] or electrothermal actuation [
40,
41,
42,
43,
44,
45,
46]. However the advent of smart materials in the last decades has greatly increased the possibilities of development of new smart structures [
47,
48,
49] with the ability, not possible for the traditional systems, to adapt to external conditions variations. Because of their speed of response, low power consumption and high operating bandwidth the piezoelectric materials are, among the smart materials, the most promising ones for active vibration control [
50,
51,
52,
53] and MEMS applications [
54]. However the research in the latter field mainly focuses on the energy harvesting [
55,
56,
57,
58] or MEMS tweezers for manipulating micro-objects and microassembly [
59,
60,
61].
This paper presents the design of a novel piezoMEMS tweezer for the analysis and characterization of soft materials. Each jaw can be built as a sandwich composite beam and activated by an electric field.
The tweezer is supposed to be force controlled because the piezoelectric materials produce a stress proportional to the applied electrical field. Furthermore, a sensor is supposed to be integrated into the structure for displacement control. This gives rise to improve the analysis of the cell properties. In fact, the displacement-controlled actuator is able to identify the beginning of a rupture during straining or softening, while a force-controlled system maintains a constant force regardless of the required displacement [
62]. The action of the piezoelectric material has been modeled by the Pin Force Model [
63].
By applying symmetric electric fields to the composite jaws, they will bend in opposite directions allowing the gripping of the soft tissue.
A new mathematical model to measure the stiffness, the equivalent Young’s modulus, and the viscous damping coefficient of the soft tissue has been developed. The model has been tested on three different soft test specimens and the results were in good agreement with those obtained by COMSOL finite elements code.
2. The Adopted Piezo-Mems Microgripper
The purpose of this paper is to develop a theoretical model of a piezo-MEMS microgripper, which can be used to characterize a grasped sample tissue. Therefore, the actual fabrication process of this instrument will not be herein considered. However, for the sake of completeness, a selection of possible materials and technological processes is briefly recalled.
In the last decades, several actuation methods have been proposed to induce motion in MEMS devices such as electrostatic, thermal and piezoelectric. The electrostatic devices are widely adopted but the piezoelectric MEMS’s offer some attractive advantages: lower power consumption, broader bandwidth and approximately ten times lower voltages to obtain the same given displacement [
64]. Furthermore the piezoelectric materials can be manufactured using the same MEMS conventional technologies and for this reason these materials have been preferred to develop piezo-MEMS devices in the last decades. Typical applications include vibration energy harvesters [
65,
66], resonators [
67], capacitors [
68], micro sensors/actuators [
69,
70], micromachined ultrasonic transducer [
71], gyroscopic sensors [
72], microlens [
73,
74], 1D and 2D micro-scanners [
75,
76].
The piezoelectric materials can be gathered into two groups: ferroelectric (lead zirconate titanate, PZT compounds) and non-ferroelectric (ZnO and AlN). The piezoelectric characteristics (piezoelectric coefficient, Q factor, dielectric constant, etc.) rely on the crystalline structure. In fact ZnO and AlN thin-films show wurtzite structure that entails lower piezoelectricity if compared to PZT materials (perovskite structure). Nevertheless ZnO and AlN exhibit large mechanical stiffness, high
Q factor and do not require a polarization process so they can be attractive for sensors applications. PZT thin-films provide high piezoelectric properties, lower cost and stability against temperature but require a poling process before using the piezo-MEMS device [
77]. The electric field poling direction depends on the functional configuration of the piezo-MEMS.
Usually the piezoelectric MEMS actuators/sensors are based on cantilever structures and the number of active layers identifies their working configuration:
When a PZT bimorph bending beam and the transverse piezoelectric effect (
) are considered, the PZT layers must be poled in opposite directions in order to maximize the bending action. Then the electrodes of the PZT layers in contact with the structural layer share the same electric potential, whereas the outer electrodes share the same opposite sign potential (see
Section 3). The design and fabrication process of piezo-MEMS devices have been extensively explored for the above mentioned unimorph and bimorph cantilever configurations using the aforementioned materials:
Various technologies can be applied to deposit piezoelectric thin-films such as
pulsed laser deposition (PLD);
chemical vapour deposition (CVD);
screen printing;
sol-gel and
radio frequency sputtering.
Usually the sol-gel and sputtering methods are the preferred ones both for research and commercial production because they allow the piezoelectric thin-film to be homogeneously deposited on large Si wafers [
79]. The beam structural layer can be metal-based or silicon-based and the etching processes could be accomplished relying on conventional techniques (RIE, D-RIE, ECR).
A schematic view of the target piezo-MEMS tweezer is qualitatively depicted in
Figure 1. The system can be obtained by using a multilayer wafer that can be built by using the above mentioned techniques. At the end of the process, the whole microsystem is composed of two bimorph beams (
a)-(
b)-(
c) (see
Figure 1). The two bimorph beams are supported by the handle layer (
d). The specific steps of the process (deposition, etching, exposure, etc.) will depend on the peculiar materials and technology selected for the construction. In the case under study, the system is conceived in such a way that the mask geometry could be quite elementary for any etching step.
Another possible layout is represented in
Figure 2, where two new layers (
e) have been added with respect to the previous example of fabrication. Layers (
a), (
b), (
c), and (
d) have the same function as described for the previous layout. The second design is better for the operational aspects because a clamping tooth has been added for each jaw. However, these two teeth are more difficult to obtain during the process because they require at least two more deposition layers (
e) and also a more complex series of intermediate etching-deposition steps that depend on the selected materials.
4. Results and Discussion
To validate the proposed model, numerical simulations have been done. The dimensions and the material characteristics are summarized in
Table 1:
In this work, the FEM results have been chosen as the reference values. Three typical soft tissues (liver, muscle and uterus) of known characteristics [
83], have been considered. The value of the beam tip displacement, obtained by the FEM simulations, has been included in the mathematical model to calculate the equivalent Young’s modulus and the viscous damping coefficient. The ST sample stiffness can be calculated by means of Equation (
8). The values of the Young’s modulus and the viscous coefficients reported in the literature have been compared with the ones calculated by the new method. Because of the symmetry of the structure with respect to its mid-plane (see
Figure 7) only the upper part has been considered in the simulations.
In
Table 2 the results of the static simulations, obtained by the above described procedure, have been reported. It is possible to observe that the model results are in good agreement with the real values with a percentage error less than
in all the cases.
To obtain the viscous damping coefficient, dynamics simulations are necessary. As described above, the chosen strategy consists in exciting, by the piezoelectric plates, the i-th mode of the structure, in order to obtain the amplitude of the free end displacement and then in including this in the model.
A comparison between the eigenfrequencies obtained by the COMSOL FEM code and the proposed model is reported in
Table 3.
In the FEM simulations the first mode has been chosen to excite the structure with the values of the electrical potential reported in
Table 4.
By neglecting the initial transient part, the axial displacements for the various soft tissues have been reported in
Figure 8.
Finally, the comparison between the actual viscous damping coefficients and those obtained by the model results have been highlighted in
Table 5.
A good agreement between the relative coefficients is also observed with a percentage error always less than .