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Appl. Sci. 2018, 8(9), 1555; https://doi.org/10.3390/app8091555

Lactobacillus acidophilus Derived Biosurfactant as a Biofilm Inhibitor: A Promising Investigation Using Microfluidic Approach

1
Department of Microbiology, Savitribai Phule Pune University, Pune 411007, Maharashtra, India
2
Department of Physics, Savitribai Phule Pune University, Pune 411007, Maharashtra, India
3
School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK
*
Authors to whom correspondence should be addressed.
Received: 31 July 2018 / Revised: 18 August 2018 / Accepted: 29 August 2018 / Published: 4 September 2018
(This article belongs to the Special Issue Nano-systems for Antimicrobial Therapy)
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Abstract

Background: Biomedical devices and implants are adversely affected by biofilm-associated infections that pose serious public health issues. Biosurfactants (BSs) can combat pathogenic biofilms through their antimicrobial, antibiofilm and antiadhesive capabilities. The objective of our research was to produce biosurfactant (BS) from Lactobacillus acidophilus NCIM 2903 and investigate its antibiofilm, antiadhesive potential using microfluidics strategies by mimicking the micro-environment of biofilm. Methods: Antibiofilm and antiadhesive potential was effectively evaluated using different methods like microfluidics assay, catheter assay, polydimethlysiloxane (PDMS) disc assay. Along with this chemical and physical characteristics of BS were also evaluated. Results: Cell free biosurfactant (CFBS) obtained was found to be effective against biofilm which was validated through the microfluidic (MF) or Lab on Chip (LOC) approach. The potency of CFBS was also evaluated on catheter tubing and PDMS surfaces (representative bioimplants). The efficacy of CFBS was also demonstrated through the reduction in surface tension, interfacial tension, contact angle and low critical micelle concentration. Conclusion: CFBS was found to be a potent antimicrobial and antibiofilm agent. We believe that perhaps this is the first report on demonstrating the inhibiting effect of Lactobacillus spp. derived CFBS against selected bacteria via LOC approach. These findings can be explored to design various BSs based formulations exhibiting antimicrobial, antibiofilm and antiadhesive potential for biomedical applications. View Full-Text
Keywords: biofilm; bioimplant; biosurfactant; Lactobacillus; microfluidics. biofilm; bioimplant; biosurfactant; Lactobacillus; microfluidics.
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Satpute, S.K.; Mone, N.S.; Das, P.; Banpurkar, A.G.; Banat, I.M. Lactobacillus acidophilus Derived Biosurfactant as a Biofilm Inhibitor: A Promising Investigation Using Microfluidic Approach. Appl. Sci. 2018, 8, 1555.

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