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The Enhancing Effects of Amelogenin Exon 5-Encoded Peptide from Enamel Matrix Derivative on Odontoblast-Like KN-3 Cells

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Department of Periodontology, Osaka Dental University, Osaka 573-1121 Japan
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Department of Endodontics, Osaka Dental University, Osaka 573-1121, Japan
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Department of Oral Pathology, Osaka Dental University, Osaka 573-1121, Japan
*
Author to whom correspondence should be addressed.
Appl. Sci. 2018, 8(10), 1890; https://doi.org/10.3390/app8101890
Received: 30 August 2018 / Accepted: 8 October 2018 / Published: 11 October 2018
(This article belongs to the Section Applied Biosciences and Bioengineering)
Enamel matrix derivative (EMD) is applied for periodontal therapy. We created a synthetic amelogenin peptide (SP) derived from EMD, and have previously investigated the biological function of SP. However, it is unknown whether SP affects odontoblastic differentiation. In this study, we tested the effects of SP in the odontoblast-like cells, KN-3 cells. KN-3 cells were cultured with SP (0 to 1000 ng/mL) and then cultured for 3, 8, 24, or 48 h in order to determine the effects of SP on cell proliferation and detect its optimum concentration. KN-3 cells were treated with SP in odontogenic differentiation medium cultured for 3 or 7 days. Odontogenic markers were measured by the detection of alkaline phosphatase (ALP) activity and dentin sialo phosphoprotein (DSPP) expression, the calcified nodule formation, and calcium deposition. The addition of SP significantly promoted cell proliferation at 100 ng/mL, generating the greatest change in cell proliferation. SP also showed increased odontogenic expression markers and mineralization. These results suggest that SP, derived from EMD, could have potential for application in dental pulp capping. View Full-Text
Keywords: emdogain; amelogenin; odontoblast; differentiation; mineralization emdogain; amelogenin; odontoblast; differentiation; mineralization
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MDPI and ACS Style

Kato, H.; Taguchi, Y.; Imai, K.; Ruan, Y.; Tsai, Y.-W.; Chen, Y.-C.; Shida, M.; Taguchi, R.; Tominaga, K.; Umeda, M. The Enhancing Effects of Amelogenin Exon 5-Encoded Peptide from Enamel Matrix Derivative on Odontoblast-Like KN-3 Cells. Appl. Sci. 2018, 8, 1890.

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