Molecular Characterization of Isolates of the Banana Bunchy Top Virus (BBTV) from the District of Chókwè, Mozambique
Abstract
:Featured Application
Abstract
1. Introduction
2. Materials and Methods
2.1. BBTV Isolates Analyzed
2.2. Amplification and Analysis of the S and R Genes
2.3. Sequencing and Analysis of S- and R-DNA Sequences
3. Results
3.1. Amplification of the S and R Genes
3.2. Analysis of the BBTV S-DNA Sequences
3.3. Analysis of the BBTV R-DNA Sequences
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer | Sequence (5′–3′) | Gene | Temp (°C) | Size (bp) |
---|---|---|---|---|
BBTV 67SF | CGTTTAGATGGGTTTGGG | DNA S | 49 | 718 |
BBTV 784SR | CGTTTAGATGGGTTTGGG | |||
BBTV 32RF | CGGGACGGGACATTTGC | DNA R | 52 | 1007 |
BBTV 1038SR | CGTTAGGATAGCACAATC |
Group * | R-DNA | ||
Origin | GenBank | Reference | |
SEA | Vietnam | AF416477 | [11] |
SEA | Vietnam | AF416472 | [11] |
SEA | Taiwan | AF416468 | [15] |
SEA | Philippines | AF416469 | [15] |
PIO | Egypt | AF416465 | [15] |
PIO | Fiji | AF416466 | [15] |
PIO | Tonga | AF416467 | [15] |
SEA | China | HQ378190 | [20] |
SEA | China | HQ616074 | [20] |
SEA | Japan | AB108458 | [25] |
SEA | Indonesia | AB186924 | [25] |
SEA | Japan | AB108455 | [25] |
SEA | Indonesia | AB186925 | [25] |
PIO | Egypt | HQ259074 | [26] |
PIO | Malawi | JQ820453 | [27] |
PIO | Rwanda | JQ820465 | [27] |
PIO | Pakistan | AM418538 | [28] |
PIO | DRC | KM607635 | [29] |
PIO | Australia | KM607623 | [29] |
PIO | Burundi | KM607598 | [29] |
OuGR | Malaysia | EF546813 | [30] |
Group * | S-DNA | ||
Origin | GenBank | Reference | |
PIO | Malawi | JF755980 | [8] |
SEA | Myanmar | AB252642 | [25] |
SEA | China | KM607536 | [29] |
SEA | Taiwan | KM607468 | [29] |
SEA | Philippines | KM607469 | [29] |
PIO | Egypt | KM607471 | [29] |
PIO | Egypt | KM607470 | [29] |
PIO | Tonga | KM607584 | [29] |
PIO | Tonga | KM607550 | [29] |
PIO | DRC | KM607505 | [29] |
PIO | Australia | KM607489 | [29] |
PIO | Burundi | KM607456 | [29] |
OuGR | Malaysia | NC_010316 | [30] |
PIO | Rwanda | JQ820467 | [31] |
SEA | Malawi | JQ820455 | [31] |
SEA | China | AF238876 | [32] |
SEA | Japan | AB078023 | [33] |
SEA | Indonesia | MT433375 | [34] |
SEA | Indonesia | MT433376 | [34] |
SEA | Myanmar | AB252644 | [35] |
PIO | Pakistan | MK140621 | [36] |
BSV Isolate Name | GenBank Accession Number | |
---|---|---|
R-DNA | S-DNA | |
BBTV 252 F1 | PP097007 | PP096947 |
BBTV 260 F1 | PP097009 | PP096949 |
BBTV 261 F1 | PP097010 | PP096950 |
BBTV 262 F1 | PP097011 | PP096951 |
BBTV 271 F1 | PP097012 | PP096952 |
BBTV 273 F1 | PP097013 | PP096953 |
BBTV 280 F2 | PP096991 | PP096931 |
BBTV 281 F2 | PP096992 | PP096932 |
BBTV 285 F2 | PP096995 | PP096935 |
BBTV 286 F2 | PP096996 | PP096936 |
BBTV 291 F2 | PP096999 | PP096939 |
BBTV 292 F2 | PP097000 | PP096940 |
BBTV 299 F2 | PP097001 | PP096941 |
BBTV 301 F2 | PP097003 | PP096943 |
BBTV 308 F2 | PP097005 | PP096945 |
BBTV 232 F3 | PP096986 | PP096926 |
BBTV 241 F3 | PP096988 | PP096928 |
BBTV 243 F3 | PP096989 | PP096929 |
BBTV 340 F4 | PP096983 | PP096923 |
BBTV 344 F4 | PP096984 | PP096924 |
BBTV 329 F5 | PP096980 | PP096920 |
BBTV 335 F5 | PP096982 | PP096922 |
BBTV 311 F7 | PP096979 | PP096919 |
BBTV 201 F8 | PP096977 | PP096917 |
BBTV 204 F8 | PP096978 | PP096918 |
BBTV 205 F9 | PP096975 | PP096915 |
BBTV 210 F9 | PP096976 | PP096916 |
BBTV 217 F10 | PP096971 | PP096911 |
BBTV 222 F10 | PP096972 | PP096912 |
BBTV 224 F10 | PP096973 | PP096913 |
BBTV 225 F10 | PP096974 | PP096914 |
BBTV 171 F11 | PP096954 | PP096894 |
BBTV 172 F11 | PP096955 | PP096895 |
BBTV 177 F11 | PP096959 | PP096899 |
BBTV 179 F11 | PP096961 | PP096901 |
BBTV 180 F11 | PP096962 | PP096902 |
BBTV 183 F11 | PP096963 | PP096903 |
BBTV 187 F11 | PP096967 | PP096907 |
BBTV 191 F11 | PP096968 | PP096908 |
BBTV 193 F11 | PP096969 | PP096909 |
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Barros, S.C.I.M.; Figueira, A.d.R.; Silveira, A.T.L. Molecular Characterization of Isolates of the Banana Bunchy Top Virus (BBTV) from the District of Chókwè, Mozambique. Appl. Sci. 2024, 14, 4291. https://doi.org/10.3390/app14104291
Barros SCIM, Figueira AdR, Silveira ATL. Molecular Characterization of Isolates of the Banana Bunchy Top Virus (BBTV) from the District of Chókwè, Mozambique. Applied Sciences. 2024; 14(10):4291. https://doi.org/10.3390/app14104291
Chicago/Turabian StyleBarros, Sandra Carvalho I. Mussa, Antonia dos Reis Figueira, and Antonia Thalyta Lopes Silveira. 2024. "Molecular Characterization of Isolates of the Banana Bunchy Top Virus (BBTV) from the District of Chókwè, Mozambique" Applied Sciences 14, no. 10: 4291. https://doi.org/10.3390/app14104291
APA StyleBarros, S. C. I. M., Figueira, A. d. R., & Silveira, A. T. L. (2024). Molecular Characterization of Isolates of the Banana Bunchy Top Virus (BBTV) from the District of Chókwè, Mozambique. Applied Sciences, 14(10), 4291. https://doi.org/10.3390/app14104291