Effects of Porcine Whole-Blood Protein Hydrolysate on Exercise Function and Skeletal Muscle Differentiation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Materials
2.2. Amino Acid Content Determination
2.3. Animals and Treatment
2.4. Exercise Performance
2.5. Cell Culture and Myotube Differentiation
2.6. Western Blotting
2.7. Histological Analysis of Muscle
2.8. Statistical Analysis
3. Results
3.1. PWBPH Supplementation Increased Muscle Mass and Exercise Performance
3.2. PWBPH Supplementation Enhanced Skeletal Muscle Differentiation In Vivo
3.3. PWBPH Supplementation Induced Differentiation and Hypertrophy of C2C12 Cells
3.4. PWBPH Supplementation Induced Akt/mTOR Signaling Pathways In Vivo and In Vitro
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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List of Amino Acid | Contents (mg/100 g) | |
---|---|---|
Physiological amino acid | Threonine | 1197.01 |
Cysteine | 70.79 | |
Tyrosine | 1111.59 | |
Arginine | - | |
Alanine | 2056.28 | |
Proline | 165.18 | |
Lysine | 1464.38 | |
Histidine | 984.23 | |
Isoleucine | 611.30 | |
Leucine | 6016.50 | |
Methionine | 401.51 | |
Phenylalanine | 2147.77 | |
Tryptophan | 76.33 | |
Valine | 1738.06 | |
Glutamic Acid | 1850.13 | |
Aspartic Acid | 1520.94 | |
Serine | 1226.45 | |
Glycine | 340.33 |
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Jin, S.W.; Lee, G.H.; Kim, J.Y.; Kim, C.Y.; Choo, Y.M.; Cho, W.; Choi, J.H.; Han, E.H.; Hwang, Y.P.; Jeong, H.G. Effects of Porcine Whole-Blood Protein Hydrolysate on Exercise Function and Skeletal Muscle Differentiation. Appl. Sci. 2022, 12, 17. https://doi.org/10.3390/app12010017
Jin SW, Lee GH, Kim JY, Kim CY, Choo YM, Cho W, Choi JH, Han EH, Hwang YP, Jeong HG. Effects of Porcine Whole-Blood Protein Hydrolysate on Exercise Function and Skeletal Muscle Differentiation. Applied Sciences. 2022; 12(1):17. https://doi.org/10.3390/app12010017
Chicago/Turabian StyleJin, Sun Woo, Gi Ho Lee, Ji Yeon Kim, Chae Yeon Kim, Young Moo Choo, Whajung Cho, Jae Ho Choi, Eun Hee Han, Yong Pil Hwang, and Hye Gwang Jeong. 2022. "Effects of Porcine Whole-Blood Protein Hydrolysate on Exercise Function and Skeletal Muscle Differentiation" Applied Sciences 12, no. 1: 17. https://doi.org/10.3390/app12010017
APA StyleJin, S. W., Lee, G. H., Kim, J. Y., Kim, C. Y., Choo, Y. M., Cho, W., Choi, J. H., Han, E. H., Hwang, Y. P., & Jeong, H. G. (2022). Effects of Porcine Whole-Blood Protein Hydrolysate on Exercise Function and Skeletal Muscle Differentiation. Applied Sciences, 12(1), 17. https://doi.org/10.3390/app12010017