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Article

Anti-Inflammatory Effects of Jakyakgamcho-Tang in IL-4- and TNF-α-Stimulated Lung Epithelial Cells and Lipopolysaccharide-Stimulated Macrophages

KM Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Korea
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Academic Editor: João Rocha
Appl. Sci. 2021, 11(22), 10569; https://doi.org/10.3390/app112210569
Received: 30 August 2021 / Revised: 3 November 2021 / Accepted: 5 November 2021 / Published: 10 November 2021
(This article belongs to the Special Issue Herbal Medicines and Their Beneficial Effect in Inflammatory Diseases)
Asthma is a chronic respiratory disease mediated by airway inflammation. Jakyakgamcho-tang (JGT), a traditional medicine, is widely subscribed to common diseases such as muscle pain and cramps in East Asian countries. Although the efficacy of JGT on peripheral neuropathy, gouty arthritis, and colitis has been reported, the effect of JGT on airway inflammation related to asthma is not clearly investigated. In this study, we aimed to evaluate the effects of JGT water extract (JGTW) on factors related to airway inflammation using the human bronchial epithelial BEAS-2B and the mouse monocyte-macrophage RAW264.7 cell lines. Furthermore, the constituents in JGTW were quantitatively and qualitatively studied for future reference of JGTW standardization. JGTW reduced the generation of several airway inflammation mediators such as eotaxins, regulated on activation normal T-cell expressed and secreted (RANTES), and matrix metalloproteinase-9, and expressions of adhesion molecules (ICAM-1 and VCAM-1), which attracts leukocytes to the site of inflammation in interleukin-4 + tumor necrosis factor-α (IT)-stimulated BEAS-2B cells. In lipopolysaccharide-stimulated RAW264.7 cells, JGTW effectively suppressed inducible nitric oxide synthase (iNOS) induction by inhibiting the MAPK and NF-κB signaling. In addition, JGTW treatment showed decreased inflammatory cells and Th2 cytokines in bronchoalveolar lavage fluid and decreased IgE levels in plasma in the OVA-induced asthmatic mice model. In the ultra-performance liquid chromatography-diode array detector-tandem mass spectrometry analysis, 24 phytochemicals were identified in JGTW, and paeoniflorin (63.971 mg/g) and glycyrrhizin (11.853 mg/g) were found to be the most abundant. These findings suggest that JGTW has anti-inflammatory effects on airway inflammation by regulating inflammatory response-related factors, possibly through MAPK and NF-κB in pulmonary epithelial cells and macrophages. View Full-Text
Keywords: Jakyakgamcho-tang; bronchial epithelial cell; macrophage; airway inflammation; UPLC-DAD-MS/MS Jakyakgamcho-tang; bronchial epithelial cell; macrophage; airway inflammation; UPLC-DAD-MS/MS
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MDPI and ACS Style

Kim, Y.J.; Jeon, W.-Y.; Lee, M.-Y.; Hwang, Y.-H.; Kim, J. Anti-Inflammatory Effects of Jakyakgamcho-Tang in IL-4- and TNF-α-Stimulated Lung Epithelial Cells and Lipopolysaccharide-Stimulated Macrophages. Appl. Sci. 2021, 11, 10569. https://doi.org/10.3390/app112210569

AMA Style

Kim YJ, Jeon W-Y, Lee M-Y, Hwang Y-H, Kim J. Anti-Inflammatory Effects of Jakyakgamcho-Tang in IL-4- and TNF-α-Stimulated Lung Epithelial Cells and Lipopolysaccharide-Stimulated Macrophages. Applied Sciences. 2021; 11(22):10569. https://doi.org/10.3390/app112210569

Chicago/Turabian Style

Kim, Yu J., Woo-Young Jeon, Mee-Young Lee, Youn-Hwan Hwang, and Jinhee Kim. 2021. "Anti-Inflammatory Effects of Jakyakgamcho-Tang in IL-4- and TNF-α-Stimulated Lung Epithelial Cells and Lipopolysaccharide-Stimulated Macrophages" Applied Sciences 11, no. 22: 10569. https://doi.org/10.3390/app112210569

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