In Vivo Evaluation of Decellularized Human Tooth Scaffold for Dental Tissue Regeneration
, Mijeong Jeon 2, Kyounga Cheon 3, Sun Ha Kim 3, Han-Sung Jung 4, Yooseok Shin 5, Chung Min Kang 1, Seong-Oh Kim 1, Hyung-Jun Choi 1, Hyo-Seol Lee 6, Ko Eun Lee 6 and Je Seon Song 1,7,*Round 1
Reviewer 1 Report
Dear Authors, thank you for submitting your paper.
The present study evaluated a decellularized human tooth as a scaffold for the PDL and pulp tissue regeneration
I congratulate the authors for this very relevant research, which will add to the dental field.
It appears well structured, correctly carried out and written without logical or factual errors
Methodological aspects are deeply cleared in the manuscript.
The topic is in line with the journal aim.
-Data reported in the Methods section are appropriate and precisely described;.
-Results are reported clearly and adequately supported by Tables.
-I suggest to the Authors to improve their reference list citing the following recent article:
https://doi.org/10.3390/healthcare9030251
The Conclusions are correctly stated and supported by the findings obtained from the present study.
According to this Reviewer’s consideration, novelty and quality of the paper, publication of the present manuscript is recommended after minor revision
Author Response
Please see the attachment
Author Response File: 
Author Response.docx
Reviewer 2 Report
The paper is well written to claim the decellularized human tooth scaffold for dental tissue engineering. However, the authors should introduce the research on scaffolds in the field of regenerative medicine.
This paper would be accepted only when all the comments are responded.
- Introduction;
Scaffolds should be described in detail because the scaffold concept is the most important in this study. The authors should be added review papers. I suggest recent references be added for readers’ better understanding.
Int. J. Mol. Sci. 2021, 22(16), 8657
Advances in Materials Science and Engineering, vol. 2019, Article ID 3429527
- Figures 3 and 6
The expression kinetics vary from gene to gene. Therefore, the time course of gene expression should be investigated. If the authors cannot, the description should be added to the discussion.
3.
How about the degradation profiles of the scaffold?
How about the cell adhesion or cytotoxicity of the scaffold?
Author Response
Please see the attachment
Author Response File: Author Response.docx
Round 2
Reviewer 2 Report
The authors responded well to all comments. I can understand the authors' opinions. I recommend the publication.
