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Volume 11
Issue 18
10.3390/app11188421
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Article

Analgesic Effect of Boiogito, a Japanese Traditional Kampo Medicine, on Post-Traumatic Knee Osteoarthritis through Inhibition of ERK1/2 Phosphorylation in the Dorsal Horn of the Spinal Cord

by
Yusuke Kunieda
1,
Takayuki Okumo
1,2,*,
Hideshi Ikemoto
1,
Naoki Adachi
1,
Midori Tanaka
1,
Taro Kimura
1,2,
Kanako Yusa
1,2,
Koji Kanzaki
2 and
Masataka Sunagawa
1
1
Department of Physiology, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
2
Department of Orthopedic Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, Yokohama City 227-8501, Japan
*
Author to whom correspondence should be addressed.
Appl. Sci. 2021, 11(18), 8421; https://doi.org/10.3390/app11188421
Submission received: 7 August 2021 / Revised: 26 August 2021 / Accepted: 27 August 2021 / Published: 10 September 2021
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Abstract

Boiogito (BO), a Japanese traditional herbal medicine, has been proven to be clinically effective against knee osteoarthritis (KOA)-associated pain. However, the therapeutic mechanism of BO remains unclear. Thus, we investigated the analgesic mechanism of BO using a rat KOA model. KOA was induced by destabilization of the medial meniscus (DMM). Rats were allocated into the following four groups: control, sham, DMM, and DMM + BO groups. Rotarod test was performed to evaluate the pain-related locomotive dysfunction. Expression of phosphorylated extracellular signal-regulated kinase1/2 (pERK1/2) in the spinal dorsal horn was examined using immunofluorescence staining and Western blotting on days 1 and 28 after DMM surgery. A mitogen-activated protein kinase inhibitor, U0126, was intrathecally injected and rotarod test and Western blotting were performed. The rotarod test revealed hampered locomotive function in the DMM group, which was significantly improved upon BO administration. The number of pERK1/2-positive cells was increased in the DMM group, whereas it was significantly decreased in the DMM + BO group. U0126 significantly inhibited ERK1/2 phosphorylation and increased walking time in the rotarod test, suggesting that the DMM-related pain was associated with ERK1/2 phosphorylation in the spinal dorsal horn. In conclusion, BO administration improved the pain-related locomotive dysfunction by suppressing ERK1/2 phosphorylation.
Keywords: osteoarthritis; destabilization of the medial meniscus (DMM); kampo; boiogito; pain; ERK osteoarthritis; destabilization of the medial meniscus (DMM); kampo; boiogito; pain; ERK

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MDPI and ACS Style

Kunieda, Y.; Okumo, T.; Ikemoto, H.; Adachi, N.; Tanaka, M.; Kimura, T.; Yusa, K.; Kanzaki, K.; Sunagawa, M. Analgesic Effect of Boiogito, a Japanese Traditional Kampo Medicine, on Post-Traumatic Knee Osteoarthritis through Inhibition of ERK1/2 Phosphorylation in the Dorsal Horn of the Spinal Cord. Appl. Sci. 2021, 11, 8421. https://doi.org/10.3390/app11188421

AMA Style

Kunieda Y, Okumo T, Ikemoto H, Adachi N, Tanaka M, Kimura T, Yusa K, Kanzaki K, Sunagawa M. Analgesic Effect of Boiogito, a Japanese Traditional Kampo Medicine, on Post-Traumatic Knee Osteoarthritis through Inhibition of ERK1/2 Phosphorylation in the Dorsal Horn of the Spinal Cord. Applied Sciences. 2021; 11(18):8421. https://doi.org/10.3390/app11188421

Chicago/Turabian Style

Kunieda, Yusuke, Takayuki Okumo, Hideshi Ikemoto, Naoki Adachi, Midori Tanaka, Taro Kimura, Kanako Yusa, Koji Kanzaki, and Masataka Sunagawa. 2021. "Analgesic Effect of Boiogito, a Japanese Traditional Kampo Medicine, on Post-Traumatic Knee Osteoarthritis through Inhibition of ERK1/2 Phosphorylation in the Dorsal Horn of the Spinal Cord" Applied Sciences 11, no. 18: 8421. https://doi.org/10.3390/app11188421

APA Style

Kunieda, Y., Okumo, T., Ikemoto, H., Adachi, N., Tanaka, M., Kimura, T., Yusa, K., Kanzaki, K., & Sunagawa, M. (2021). Analgesic Effect of Boiogito, a Japanese Traditional Kampo Medicine, on Post-Traumatic Knee Osteoarthritis through Inhibition of ERK1/2 Phosphorylation in the Dorsal Horn of the Spinal Cord. Applied Sciences, 11(18), 8421. https://doi.org/10.3390/app11188421

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