Exploitation of Enzymes for the Production of Biofuels: Electrochemical Determination of Kinetic Parameters of LPMOs
Abstract
:1. Introduction
2. Materials and Methods
3. Results
3.1. Cyclic Voltammetry
3.2. FT Alternating Current Voltammetry
- A harmonic h is chosen, being free of capacitance currents. This harmonic is recorded at a fixed amplitude and decreasing frequencies;
- The limiting value of the normalized peak height, , of the principal peak is determined. For this frequency, f, the reaction is at the reversible region;
- If the transfer coefficient is to be determined, the shift in the potential of the principal peak is plotted as a function of the logarithm of the inverse frequency, . Linear regression is performed in the region of high frequencies and the transfer coefficient is determined;
- The principal peak height of a quasi-reversible harmonic is determined and the value is calculated;
- The kinetic constant is determined from the graph representing the dependance of on .
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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/s | /V |
---|---|
100 | 0.000 |
20 | 0.008 |
10 | 0.014 |
5 | 0.026 |
2 | 0.059 |
1 | 0.098 |
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Zouraris, D.; Karnaouri, A.; Xydou, R.; Topakas, E.; Karantonis, A. Exploitation of Enzymes for the Production of Biofuels: Electrochemical Determination of Kinetic Parameters of LPMOs. Appl. Sci. 2021, 11, 4715. https://doi.org/10.3390/app11114715
Zouraris D, Karnaouri A, Xydou R, Topakas E, Karantonis A. Exploitation of Enzymes for the Production of Biofuels: Electrochemical Determination of Kinetic Parameters of LPMOs. Applied Sciences. 2021; 11(11):4715. https://doi.org/10.3390/app11114715
Chicago/Turabian StyleZouraris, Dimitrios, Anthi Karnaouri, Raphaela Xydou, Evangelos Topakas, and Antonis Karantonis. 2021. "Exploitation of Enzymes for the Production of Biofuels: Electrochemical Determination of Kinetic Parameters of LPMOs" Applied Sciences 11, no. 11: 4715. https://doi.org/10.3390/app11114715