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Article

Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond

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Allmicroalgae Natural Products S.A., Research and Development Department—Rua 25 de Abril, nº 19, 2445-413 Pataias, Portugal
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MARE—Marine and Environmental Sciences Centre, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal
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CCMAR—Centre of Marine Sciences, University of Algarve, Gambelas, 8005-139 Faro, Portugal
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Global Health and Tropical Medicine (GHMT), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, 1349-008 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(9), 3040; https://doi.org/10.3390/app10093040
Received: 23 March 2020 / Revised: 19 April 2020 / Accepted: 23 April 2020 / Published: 27 April 2020
(This article belongs to the Special Issue Physiology and Biotechnology of Microalgae)
Bioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblystomatis strain. However, as our phylogenetic data strongly suggests that the aforementioned taxon belongs to the genus Chlorococcum, it is here proposed to rename this species as Chlorococcum amblystomatis. In order to characterize the biotechnological potential of this novel isolate, growth performance and biochemical composition were evaluated from the pilot (2.5-m3) to industrial (10-m3) scale. The highest maximum areal productivity (36.56 g·m−2·day−1) was reached in a 10-m3 tubular photobioreactor (PBR), as compared to that obtained in a 2.5-m3 PBR (26.75 g·m−2·day−1). Chlorococcum amblystomatis displayed high protein content (48%–56% dry weight (DW)) and moderate levels of total lipids (18%–31% DW), carbohydrates (6%–18% DW) and ashes (9%–16% DW). Furthermore, the lipid profile was dominated by polyunsaturated fatty acids (PUFAs). The highest pigment contents were obtained in the 2.5-m3 PBR, where total chlorophylls accounted for 40.24 mg·g−1 DW, followed by lutein with 5.37 mg·g−1 DW. Overall, this free-living Chlorococcum amblystomatis strain shows great potential for nutritional applications, coupling a promising growth performance with a high protein content as well as relevant amounts of PUFAs, chlorophyll, and carotenoids. View Full-Text
Keywords: bioprospection; microalgae; Chlorococcum (Oophila) amblystomatis; photobioreactors; industrial-scale production; biotechnological applications bioprospection; microalgae; Chlorococcum (Oophila) amblystomatis; photobioreactors; industrial-scale production; biotechnological applications
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MDPI and ACS Style

Correia, N.; Pereira, H.; Silva, J.T.; Santos, T.; Soares, M.; Sousa, C.B.; Schüler, L.M.; Costa, M.; Varela, J.; Pereira, L.; Silva, J. Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond. Appl. Sci. 2020, 10, 3040. https://doi.org/10.3390/app10093040

AMA Style

Correia N, Pereira H, Silva JT, Santos T, Soares M, Sousa CB, Schüler LM, Costa M, Varela J, Pereira L, Silva J. Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond. Applied Sciences. 2020; 10(9):3040. https://doi.org/10.3390/app10093040

Chicago/Turabian Style

Correia, Nádia, Hugo Pereira, Joana T. Silva, Tamára Santos, Maria Soares, Carolina B. Sousa, Lisa M. Schüler, Margarida Costa, João Varela, Leonel Pereira, and Joana Silva. 2020. "Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond" Applied Sciences 10, no. 9: 3040. https://doi.org/10.3390/app10093040

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