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Article

Tympanic Membrane Collagen Expression by Dynamically Cultured Human Mesenchymal Stromal Cell/Star-Branched Poly(ε-Caprolactone) Nonwoven Constructs

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Department of Clinical and Experimental Medicine, University of Pisa, via Savi 10, 56126 Pisa, Italy
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Department of Surgical, Medical, Molecular Pathology and Emergency Medicine, University of Pisa, via Savi 10, 56126 Pisa, Italy
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Department of Chemistry and Industrial Chemistry, University of Pisa, via G. Moruzzi 13, 56124 Pisa, Italy
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Neuroscience Centre, University “Magna Graecia”, viale Europa, 88100 Catanzaro, Italy
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The Biorobotics Institute, Scuola Superiore Sant’Anna, via R. Piaggio 34, 56025 Pontedera (PI), Italy
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Department of Civil and Industrial Engineering, University of Pisa, Largo L. Lazzarino 2, 56122 Pisa, Italy
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(9), 3043; https://doi.org/10.3390/app10093043
Received: 24 March 2020 / Revised: 22 April 2020 / Accepted: 23 April 2020 / Published: 27 April 2020
(This article belongs to the Special Issue New Trends in Bioresorbable Polymers for Biomedical Applications)
The tympanic membrane (TM) primes the sound transmission mechanism due to special fibrous layers mainly of collagens II, III, and IV as a product of TM fibroblasts, while type I is less represented. In this study, human mesenchymal stromal cells (hMSCs) were cultured on star-branched poly(ε-caprolactone) (*PCL)-based nonwovens using a TM bioreactor and proper differentiating factors to induce the expression of the TM collagen types. The cell cultures were carried out for one week under static and dynamic conditions. Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) were used to assess collagen expression. A Finite Element Model was applied to calculate the stress distribution on the scaffolds under dynamic culture. Nanohydroxyapatite (HA) was used as a filler to change density and tensile strength of *PCL scaffolds. In dynamically cultured *PCL constructs, fibroblast surface marker was overexpressed, and collagen type II was revealed via IHC. Collagen types I, III and IV were also detected. Von Mises stress maps showed that during the bioreactor motion, the maximum stress in *PCL was double that in HA/*PCL scaffolds. By using a *PCL nonwoven scaffold, with suitable physico-mechanical properties, an oscillatory culture, and proper differentiative factors, hMSCs were committed into fibroblast lineage-producing TM-like collagens. View Full-Text
Keywords: eardrum; electrospinning; star-branched; poly(ε-caprolactone); bioreactor; fibroblast differentiation; collagen type II; mesenchymal stem cells; tympanoplasty; biomimetic eardrum; electrospinning; star-branched; poly(ε-caprolactone); bioreactor; fibroblast differentiation; collagen type II; mesenchymal stem cells; tympanoplasty; biomimetic
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MDPI and ACS Style

Moscato, S.; Rocca, A.; D’Alessandro, D.; Puppi, D.; Gramigna, V.; Milazzo, M.; Stefanini, C.; Chiellini, F.; Petrini, M.; Berrettini, S.; Danti, S. Tympanic Membrane Collagen Expression by Dynamically Cultured Human Mesenchymal Stromal Cell/Star-Branched Poly(ε-Caprolactone) Nonwoven Constructs. Appl. Sci. 2020, 10, 3043. https://doi.org/10.3390/app10093043

AMA Style

Moscato S, Rocca A, D’Alessandro D, Puppi D, Gramigna V, Milazzo M, Stefanini C, Chiellini F, Petrini M, Berrettini S, Danti S. Tympanic Membrane Collagen Expression by Dynamically Cultured Human Mesenchymal Stromal Cell/Star-Branched Poly(ε-Caprolactone) Nonwoven Constructs. Applied Sciences. 2020; 10(9):3043. https://doi.org/10.3390/app10093043

Chicago/Turabian Style

Moscato, Stefania, Antonella Rocca, Delfo D’Alessandro, Dario Puppi, Vera Gramigna, Mario Milazzo, Cesare Stefanini, Federica Chiellini, Mario Petrini, Stefano Berrettini, and Serena Danti. 2020. "Tympanic Membrane Collagen Expression by Dynamically Cultured Human Mesenchymal Stromal Cell/Star-Branched Poly(ε-Caprolactone) Nonwoven Constructs" Applied Sciences 10, no. 9: 3043. https://doi.org/10.3390/app10093043

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