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Article

Lab-Scale Optimization of Aurantiochytrium sp. Culture Medium for Improved Growth and DHA Production

1
Allmicroalgae Natural Products, S.A., Rua 25 de Abril, n° 19, 2445-411 Pataias, Portugal
2
CCMAR—Centre of Marine Sciences, University of Algarve, Gambelas, 8005-139 Faro, Portugal
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(7), 2500; https://doi.org/10.3390/app10072500
Received: 18 February 2020 / Revised: 29 March 2020 / Accepted: 31 March 2020 / Published: 5 April 2020
(This article belongs to the Special Issue Physiology and Biotechnology of Microalgae)
Thraustochytrids have gained increasing relevance over the last decades, due to their fast growth and outstanding capacity to accumulate polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA). In this context, the present work aimed to optimize the growth performance and DHA yields by improving the culture medium of Aurantiochytrium sp. AF0043. Accordingly, two distinct culture media were optimized: (i) an inorganic optimized medium (IOM), containing only monosodium glutamate and glucose as nitrogen and carbon sources, respectively; and (ii) an organic and sustainable waste-based optimized medium (WOM), containing corn steep powder and glycerol, added in fed-batch mode, as nitrogen and carbon sources, respectively. Overall, the lab-scale optimization allowed to increase the biomass yield 1.5-fold and enhance DHA content 1.7-fold using IOM. Moreover, WOM enabled a 2-fold increase in biomass yield and a significant improvement in lipid contents, from 22.78% to 31.14%. However, DHA content was enhanced almost 3-fold, from an initial content of 10.12% to 29.66% of total fatty acids contained in the biomass. Therefore, these results strongly suggest, not only that the production pipeline was significantly improved but also confirmed the potential use of Aurantiochytrium sp. AF0043 as a source of DHA. View Full-Text
Keywords: thraustochytrids; Aurantiochytrium sp.; docosahexaenoic acid (DHA); culture medium optimization; growth performance thraustochytrids; Aurantiochytrium sp.; docosahexaenoic acid (DHA); culture medium optimization; growth performance
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MDPI and ACS Style

Trovão, M.; Pereira, H.; Costa, M.; Machado, A.; Barros, A.; Soares, M.; Carvalho, B.; Silva, J.T.; Varela, J.; Silva, J. Lab-Scale Optimization of Aurantiochytrium sp. Culture Medium for Improved Growth and DHA Production. Appl. Sci. 2020, 10, 2500. https://doi.org/10.3390/app10072500

AMA Style

Trovão M, Pereira H, Costa M, Machado A, Barros A, Soares M, Carvalho B, Silva JT, Varela J, Silva J. Lab-Scale Optimization of Aurantiochytrium sp. Culture Medium for Improved Growth and DHA Production. Applied Sciences. 2020; 10(7):2500. https://doi.org/10.3390/app10072500

Chicago/Turabian Style

Trovão, Mafalda, Hugo Pereira, Margarida Costa, Adriana Machado, Ana Barros, Maria Soares, Bernardo Carvalho, Joana T. Silva, João Varela, and Joana Silva. 2020. "Lab-Scale Optimization of Aurantiochytrium sp. Culture Medium for Improved Growth and DHA Production" Applied Sciences 10, no. 7: 2500. https://doi.org/10.3390/app10072500

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