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Open AccessArticle

Simultaneous Quantification of Eight Marker Components in Traditional Herbal Formula, Haepyoyijin-Tang Using HPLC–PDA

Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea
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Appl. Sci. 2020, 10(11), 3888; https://doi.org/10.3390/app10113888
Received: 13 May 2020 / Revised: 29 May 2020 / Accepted: 1 June 2020 / Published: 3 June 2020
(This article belongs to the Special Issue Biomedical Application of Plant Extracts)
Haepyoyijin-tang (HPYJT) is a traditional herbal prescription that is composed of 12 medicinal herbs. Although HPYJT is frequently used in patients with asthma in Korea, no quality assessment protocols have been developed. In the present study, qualitative and quantitative analyses were performed using high-performance liquid chromatography and liquid chromatography–tandem mass spectrometry on the eight main components (mulberroside A, amygalin, liquiritin apioside, liquiritin, narirutin, hesperidin, rosmarinic acid, and glycyrrhizinic acid) to establish a quality control protocol for HPYJT. The simultaneous analysis method developed was satisfactorily validated with respect to linearity, limit of detection, limit of quantification, recovery, and precision. This analytical method thus provides an efficient approach for the evaluation of HPYJT quality. View Full-Text
Keywords: simultaneous quantification; Haepyoyijin-tang; HPLC–PDA simultaneous quantification; Haepyoyijin-tang; HPLC–PDA
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Seo, C.-S.; Lee, M.-Y. Simultaneous Quantification of Eight Marker Components in Traditional Herbal Formula, Haepyoyijin-Tang Using HPLC–PDA. Appl. Sci. 2020, 10, 3888.

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