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Article

Effect of Prooxidative Natural Products: Comparison of the OSI1 (YKL071w) Promoter Luciferase Construct from Yeast with an Nrf2/Keap Reporter System

1
Department of Plant Physiology (Bio III), RWTH Aachen University, Worringer Weg 1, 52056 Aachen, Germany
2
Leibniz Institute for Natural Product Research and Infection Biology, Beutenbergstraße 11a, 07745 Jena, Germany
3
Department of Anatomy and Cell Biology, Uniklinik RWTH Aachen University, 52056 Aachen, Germany
*
Author to whom correspondence should be addressed.
These authors contribute equally to this work.
Appl. Sci. 2020, 10(10), 3520; https://doi.org/10.3390/app10103520
Received: 21 April 2020 / Revised: 12 May 2020 / Accepted: 12 May 2020 / Published: 20 May 2020
(This article belongs to the Section Applied Biosciences and Bioengineering)
The oxidative stress response (OSR) in yeast is under the control of oxidation-sensitive cysteines in the Yap1p transcription factor, and fusion of the Yap1p-dependent OS-induced promoter of the YKL071w gene (OSI1) to a luciferase coding sequence makes a sensitive reporter for OS induced by electrophiles. In mammalian cells, the OSR induced by electrophiles is coordinated in a mechanistically similar way via oxidation-sensitive cysteines in the kelch-like ECH-associated protein 1 (Keap1)– nuclear factor erythroid 2-related factor 2 / antioxidant response element ( Nrf2/ARE) system. Many electrophilic oxidants have already been independently shown to trigger both the Yap1 and Keap1 systems. Here, we investigated the responses of Yap1 and Keap1 reporters to sulforaphane (SFN), allyl isothiocyanate (AITC), phenylethyl isothiocyanate (PEITC), previously known to stimulate Keap1–Nrf2/ARE but not known to activate Yap1, and as a positive control, allicin, previously reported to stimulate both Yap1 and Nrf2. We have compared the reciprocal responsiveness of the respective reporter systems and show that the yeast reporter system can have predictive value for electrophiles that stimulate the mammalian Keap1–Nrf2/ARE system. View Full-Text
Keywords: Yap1p; YKL071w; luciferase; allicin; phenylethyl isothiocyanate; allyl isothiocyanate; sulforaphane; monomethyl fumarate; diethyl fumarate; kavalactones; hepatocytes; electrophile; oxidative stress; antioxidant; phase II protective enzymes Yap1p; YKL071w; luciferase; allicin; phenylethyl isothiocyanate; allyl isothiocyanate; sulforaphane; monomethyl fumarate; diethyl fumarate; kavalactones; hepatocytes; electrophile; oxidative stress; antioxidant; phase II protective enzymes
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MDPI and ACS Style

Schlembach, I.; Uebachs, A.; Caspers, T.; Fragoulis, A.; Slusarenko, A.J.; Gruhlke, M.C.H. Effect of Prooxidative Natural Products: Comparison of the OSI1 (YKL071w) Promoter Luciferase Construct from Yeast with an Nrf2/Keap Reporter System. Appl. Sci. 2020, 10, 3520. https://doi.org/10.3390/app10103520

AMA Style

Schlembach I, Uebachs A, Caspers T, Fragoulis A, Slusarenko AJ, Gruhlke MCH. Effect of Prooxidative Natural Products: Comparison of the OSI1 (YKL071w) Promoter Luciferase Construct from Yeast with an Nrf2/Keap Reporter System. Applied Sciences. 2020; 10(10):3520. https://doi.org/10.3390/app10103520

Chicago/Turabian Style

Schlembach, Ivan, Andreas Uebachs, Tim Caspers, Athanassios Fragoulis, Alan J. Slusarenko, and Martin C.H. Gruhlke 2020. "Effect of Prooxidative Natural Products: Comparison of the OSI1 (YKL071w) Promoter Luciferase Construct from Yeast with an Nrf2/Keap Reporter System" Applied Sciences 10, no. 10: 3520. https://doi.org/10.3390/app10103520

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