Transcriptional Regulation of the Bovine Fatty Acid Transport Protein 1 Gene by Krüppel-Like Factors 15
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Tissue Expression Profiling
2.2. Rapid Amplification of cDNA Ends (5′ RACE)
2.3. Construct Generation
2.4. Cell Culture and Transfection
2.5. Electrophoretic Mobility Shift Assays (EMSA)
3. Results
3.1. Assessment of the Expression of SLC27A1 in Bovine Tissues
3.2. SLC27A1 TSS Identification
3.3. SLC27A1 Promoter Sequencing and Isolation
3.4. KLF15 Activates the Core SLC27A1 Promoter
3.5. KLF15 Binds to the SLC27A1 Promoter
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Zhao, Z.; Tian, H.; Shi, B.; Jiang, Y.; Liu, X.; Hu, J. Transcriptional Regulation of the Bovine Fatty Acid Transport Protein 1 Gene by Krüppel-Like Factors 15. Animals 2019, 9, 654. https://doi.org/10.3390/ani9090654
Zhao Z, Tian H, Shi B, Jiang Y, Liu X, Hu J. Transcriptional Regulation of the Bovine Fatty Acid Transport Protein 1 Gene by Krüppel-Like Factors 15. Animals. 2019; 9(9):654. https://doi.org/10.3390/ani9090654
Chicago/Turabian StyleZhao, Zhidong, Hongshan Tian, Bingang Shi, Yanyan Jiang, Xiu Liu, and Jiang Hu. 2019. "Transcriptional Regulation of the Bovine Fatty Acid Transport Protein 1 Gene by Krüppel-Like Factors 15" Animals 9, no. 9: 654. https://doi.org/10.3390/ani9090654