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Open AccessArticle

Early Pregnancy Induces Expression of STAT1, OAS1 and CXCL10 in Ovine Spleen

Department of Animal Science, College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056021, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Animals 2019, 9(11), 882; https://doi.org/10.3390/ani9110882
Received: 20 September 2019 / Revised: 25 October 2019 / Accepted: 26 October 2019 / Published: 30 October 2019
(This article belongs to the Special Issue Reproductive Management of Sheep and Goats)
Interferon-tau is a maternal recognition factor in ruminants, and spleen plays an essential role in regulating innate and adaptive immune responses. We found that interferon-tau derived from conceptus induces expression of STAT1, OAS1, and CXCL10 in ovine maternal spleen, which may be helpful for maternal immune regulation.
Interferon-tau is a maternal recognition factor in ruminant species, and spleen plays an essential role in regulating innate and adaptive immune responses. However, it is not fully understood that early pregnancy induces expression of interferon stimulated genes (ISGs) in the spleen during early pregnancy in ewes. In this study, spleens were collected from ewes at day 16 of the estrous cycle, and on days 13, 16, and 25 of gestation (n = 6 for each group), and RT-qPCR, western blot and immunohistochemistry analysis were used to detect the expression of signal transducer and activator of transcription 1 (STAT1), 2′,5′-oligoadenylate synthetase 1 (OAS1), myxovirusresistance protein 1 (Mx1) and C-X-C motif chemokine 10 (CXCL10). The results revealed that STAT1, OAS1 and CXCL10 mRNA and proteins were upregulated in the spleens during early pregnancy, and STAT1 protein was located in connective tissue cells in the capsule and trabeculae, and blood cells and lymphocytes in the red pulp. However, early pregnancy had no significant effects on expression of MX1 mRNA and protein. In conclusion, early pregnancy induces expression of STAT1, OAS1 and CXCL10 in maternal spleen, suggesting that maternal spleen is involved in immune regulation of pregnancy in sheep. View Full-Text
Keywords: interferon stimulated genes; pregnancy; spleen; sheep interferon stimulated genes; pregnancy; spleen; sheep
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Wang, Y.; Han, X.; Zhang, L.; Cao, N.; Cao, L.; Yang, L. Early Pregnancy Induces Expression of STAT1, OAS1 and CXCL10 in Ovine Spleen. Animals 2019, 9, 882.

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