Next Article in Journal
Compassionate Conservation: Exploring the Lives of African Wild Dogs (Lycaon pictus) in Botswana
Previous Article in Journal
Significance of Group Composition for the Welfare of Pastured Horses
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Animals 2019, 9(1), 15; https://doi.org/10.3390/ani9010015

Variation in the Caprine KAP24-1 Gene Affects Cashmere Fibre Diameter

1,2
,
1,2,3
,
1,2
,
1,2
,
1,2,3
,
1,2
,
1,2,* and 1,2,3,*
1
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
2
International Wool Research Institute, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
3
Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
*
Authors to whom correspondence should be addressed.
Received: 22 November 2018 / Revised: 10 December 2018 / Accepted: 29 December 2018 / Published: 5 January 2019
(This article belongs to the Section Animal Genetics and Genomics)
Full-Text   |   PDF [1159 KB, uploaded 5 January 2019]   |  
  |   Review Reports

Simple Summary

The keratin-associated proteins (KAPs) are structural components of cashmere fibres. The human and sheep orthologous gene encoding high-sulphur (HS)-KAP24-1 is now described for the goat species. Our study identified the caprine KAP24-1 gene on goat chromosome 1, and we found that the gene was polymorphic and that variation in the gene affected cashmere fibre diameter.

Abstract

The keratin-associated proteins (KAPs) are structural components of cashmere fibres. The gene encoding the high-sulphur (HS)-KAP24-1 (KRTAP24-1) has been identified in humans and sheep, but it has not been described in goats. In this study, we report the identification of caprine KRTAP24-1, describe variation in this gene, and investigate the effect of this variation on cashmere traits. A search for sequences orthologous to the ovine gene in the goat genome revealed a 774 bp open reading frame on chromosome 1, which could encode an HS-KAP. Based on this goat genome sequence and comparison with ovine KRTAP24-1 sequences, polymerase chain reaction (PCR) primers were designed to amplify an 856 bp fragment that would contain the entire coding region of the putative caprine KRTAP24-1. Use of this PCR amplification with subsequent single-strand conformation polymorphism (SSCP) analysis of the amplicons identified four distinct patterns of DNA bands on gel electrophoresis, with these representing four different DNA sequences (A to D), in 340 Longdong cashmere goats reared in China. The variant sequences had the highest similarity to KRTAP24-1 sequences from sheep and humans, suggesting that they are variants of caprine KRTAP24-1. Nine single-nucleotide polymorphisms (SNPs) were detected in the gene, including four non-synonymous SNPs and an SNP in proximity to the ATG start codon. Of the three common genotypes (AA, AB, and BB) found in these Longdong cashmere goats, cashmere fibres from goats of genotype AA had lower mean fibre diameter (MFD) than did those of genotype AB, and cashmere fibres from goats of genotype AB had lower MFD than did those from goats of genotype BB. View Full-Text
Keywords: keratin associated protein 24-1 (KAP24-1) gene; variation; cashmere; fibre diameter; goat keratin associated protein 24-1 (KAP24-1) gene; variation; cashmere; fibre diameter; goat
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Wang, J.; Zhou, H.; Luo, Y.; Zhao, M.; Gong, H.; Hao, Z.; Hu, J.; Hickford, J.G. Variation in the Caprine KAP24-1 Gene Affects Cashmere Fibre Diameter. Animals 2019, 9, 15.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Animals EISSN 2076-2615 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top