Application of Environmental DNA for Assessing the Distribution and Biomass of Brachymystax lenok Tsinlingensis in the Zhouzhi Heihe River
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Water Sample Collection and Environmental DNA Extraction
2.1.1. Culture Tank Experiments
2.1.2. Field Experiments
2.1.3. Environmental DNA Extraction
2.2. Establishment of qPCR Assay
3. Results
3.1. Results of Preparation of Standard Curve and LOD and LOQ Values
3.2. Results of Water Sample Experiment in Culture Tank
3.3. Estimated B. lenok Tsinlingensis Biomass in the Zhouzhi Heihe River
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Serial Number | Location | Coordinates | Altitudes |
---|---|---|---|
1 | Ban Fangzi River | 33.49103 N, 108.00369 E | 1093 m |
2 | Ba Mugou River | 33.53463 N, 107.55166 E | 1069 m |
3 | Qing Shui River | 33.50553 N, 108.03053 E | 1060 m |
4 | Yu Dongquan | 33.53253 N, 108.00194 E | 940 m |
5 | Wang Jia River-1 | 33.52363 N, 108.11431 E | 951 m |
6 | Wang Jia River-2 | 33.54246 N, 108.09191 E | 849 m |
Serial Number | Location | Number | Total Length (cm) | Body Length (cm) | Weight (g) |
---|---|---|---|---|---|
1 | Wang Jia River-1 | 1 | 14.3 | 12.4 | 21.2 |
2 | Wang Jia River-2 | 1 | 25.6 | 22.1 | 158.9 |
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Zhao, H.; Zhang, H.; Zhang, K.; Deng, J.; Fang, C.; Zhang, J.; Kong, F.; Jiang, W.; Wang, Q.; Ma, H. Application of Environmental DNA for Assessing the Distribution and Biomass of Brachymystax lenok Tsinlingensis in the Zhouzhi Heihe River. Animals 2025, 15, 977. https://doi.org/10.3390/ani15070977
Zhao H, Zhang H, Zhang K, Deng J, Fang C, Zhang J, Kong F, Jiang W, Wang Q, Ma H. Application of Environmental DNA for Assessing the Distribution and Biomass of Brachymystax lenok Tsinlingensis in the Zhouzhi Heihe River. Animals. 2025; 15(7):977. https://doi.org/10.3390/ani15070977
Chicago/Turabian StyleZhao, Hu, Han Zhang, Kunyang Zhang, Jie Deng, Cheng Fang, Jianlu Zhang, Fei Kong, Wei Jiang, Qijun Wang, and Hongying Ma. 2025. "Application of Environmental DNA for Assessing the Distribution and Biomass of Brachymystax lenok Tsinlingensis in the Zhouzhi Heihe River" Animals 15, no. 7: 977. https://doi.org/10.3390/ani15070977
APA StyleZhao, H., Zhang, H., Zhang, K., Deng, J., Fang, C., Zhang, J., Kong, F., Jiang, W., Wang, Q., & Ma, H. (2025). Application of Environmental DNA for Assessing the Distribution and Biomass of Brachymystax lenok Tsinlingensis in the Zhouzhi Heihe River. Animals, 15(7), 977. https://doi.org/10.3390/ani15070977