A20 Alleviates the Inflammatory Response in Bovine Endometrial Epithelial Cells by Promoting Autophagy
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Antibodies and Reagents
2.2. Animal Treatments
2.3. BEECs Isolation and Treatments
2.4. Histological Assay
2.5. Immunohistochemistry (IHC) Assay
2.6. Quantitative Reverse Transcriptase-PCR (qRT-PCR)
2.7. Western Blotting
2.8. Immunofluorescence Staining
2.9. Statistical Analyses
3. Results
3.1. Inflammatory Damage in the Endometrium of Dairy Cows Was Caused by E. coli
3.2. Upregulated Expression of A20 in E. coli-Infected Endometrium and LPS-Stimulated BEECs
3.3. Inhibition of Autophagy Exacerbated Inflammatory Response of BEECs Induced by LPS
3.4. A20 Inhibited LPS-Induced Inflammatory Responses in BEECs
3.5. A20 Inhibited the NF-κB Pathway Activation by Promoting Autophagy
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primers (5′→3′) | Product Size (bp) | Accession Number |
---|---|---|---|
β-actin | F: CATCACCATCGGCAATGAGC R: AGCACCGTGTTGGCGTAGAG | 156 | NM_173979.3 |
A20 | F: TGCTGCAAAGTTGGATGAAG R: TTGGGACTTTCGTTTGGTTC | 281 | XM_005210987.2 |
TNF-α | F: GGGCTTTACCTCATCTACTCACAG R: GATGGCAGACAGGATGTTGACC | 132 | NM_173966.3 |
IL-6 | F: TGAAAGCAGCAAGGAGACACT R: TGATTGAACCCAGATTGGAAGC | 90 | NM_173923.2 |
IL-8 | F: TTCCTCAGTAAAGATGCCAATG R: TGACAACCCTACACCAGACCCA | 86 | NM_173925.2 |
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Dong, J.; Ji, B.; Jiang, Y.; Fei, F.; Guo, L.; Liu, K.; Cui, L.; Meng, X.; Li, J.; Wang, H. A20 Alleviates the Inflammatory Response in Bovine Endometrial Epithelial Cells by Promoting Autophagy. Animals 2024, 14, 2876. https://doi.org/10.3390/ani14192876
Dong J, Ji B, Jiang Y, Fei F, Guo L, Liu K, Cui L, Meng X, Li J, Wang H. A20 Alleviates the Inflammatory Response in Bovine Endometrial Epithelial Cells by Promoting Autophagy. Animals. 2024; 14(19):2876. https://doi.org/10.3390/ani14192876
Chicago/Turabian StyleDong, Junsheng, Bowen Ji, Yeqi Jiang, Fan Fei, Long Guo, Kangjun Liu, Luying Cui, Xia Meng, Jianji Li, and Heng Wang. 2024. "A20 Alleviates the Inflammatory Response in Bovine Endometrial Epithelial Cells by Promoting Autophagy" Animals 14, no. 19: 2876. https://doi.org/10.3390/ani14192876
APA StyleDong, J., Ji, B., Jiang, Y., Fei, F., Guo, L., Liu, K., Cui, L., Meng, X., Li, J., & Wang, H. (2024). A20 Alleviates the Inflammatory Response in Bovine Endometrial Epithelial Cells by Promoting Autophagy. Animals, 14(19), 2876. https://doi.org/10.3390/ani14192876