The Feasibility of Animal-Based Indicators of Consciousness and Unconsciousness for Stunning in Sheep: A Systematic Review
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
3. Results and Discussion
3.1. Breathing
3.2. Posture
3.3. Tonic–Clonic Seizure
3.4. Corneal Reflex
3.5. Palpebral Reflex
3.6. Spontaneous Blinking
3.7. Muscle Tone
3.8. Eye Movements
3.9. General Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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ABMs | Description | Stunning Method |
---|---|---|
breathing | Effective stunning will result in the immediate onset of apnea (absence of breathing). Ineffectively stunned animals and those recovering consciousness will start to breathe in a pattern commonly referred to as rhythmic breathing, which involves a respiratory cycle of inspiration and expiration. Rhythmic breathing can be recognized from regular movement of the flank and/or mouth and nostrils. | captive bolt/electrical |
posture | Effective stunning will result in immediate collapse or loss of posture in animals that are not restrained or prevented from doing so. Ineffectively stunned animals, on the other hand, will fail to collapse or will attempt to regain posture after collapse. | captive bolt/electrical |
corneal reflex | The corneal reflex is elicited by touching or tapping the cornea. Ineffectively stunned animals and those recovering consciousness will blink in response to the stimulus. Unconscious animals may also intermittently show a positive corneal reflex. | captive bolt/electrical |
palpebral reflex | The palpebral reflex is elicited by touching or tapping a finger on the inner/outer eye canthus or eyelashes. Correctly stunned animals will not show a palpebral reflex. Ineffectively stunned animals and those recovering consciousness will blink in response to the stimulus. | captive bolt/electrical |
eye movements | Eye movements, including nystagmus (spontaneous rapid side-to-side movements of the eyeballs) or rotation of the eyeball indicate ineffective stunning, as effectively stunned animals will exhibit fixed eyes. | captive bolt |
vocalizations | Conscious animals may vocalize (bleating in goats and vocalization in lambs (Goldberg, 2018), and therefore purposeful vocalization can be used to recognize ineffective stunning or recovery of consciousness after stunning. However, not all conscious animals may vocalize. | captive bolt/electrical |
spontaneous blinking | Conscious animals may show spontaneous blinking, in which the animal opens/closes eyelid on its own (fast or slow) without stimulation; therefore, this sign can be used to recognize ineffective stunning or recovery of consciousness after electrical stunning. However, not all the conscious animals may show spontaneous blinking. | captive bolt/electrical |
tonic–clonic seizure | Effective head-only electrical stunning leads to the onset of tonic–clonic seizures soon after immediate collapse of the animal. The tonic seizure, which may be recognized from the tetanus, lasts for several seconds and is followed by clonic seizures lasting for seconds and leading to the loss of muscle tone. | electrical |
muscular tone | Stunned animals will show general loss of muscle tone coinciding with the recovery of breathing and the corneal reflex if not previously stuck. Loss of muscle tone can be recognized from completely relaxed legs, floppy ears and tail, and relaxed jaws with protruding tongue. Ineffectively stunned animals and those recovering consciousness will show a righting reflex and attempts to raise the head. | captive bolt |
ABMs | Outcome | Feasibility | Feasibility Score 1 |
---|---|---|---|
Breathing | TESTED questionnaire (n = 20) | ||
Apnea. | easy: 40% | 0.25 | |
normal: 45% | |||
TESTED questionnaire (n = 16) | |||
Posture | Immediate collapse. | easy: 88% | 0.81 |
normal: 6% | |||
TESTED questionnaire (n = 10) | |||
Corneal Reflex | No corneal reflex. | easy: 40% | 0.2 |
TESTED questionnaire (n = 4) | |||
Palpebral reflex | No palpebral reflex. | easy: 25% | 0 |
normal: 50% | |||
TESTED questionnaire (n = 9) | |||
Eye movements | Fixed eyes (eyes wide open and glassy). | easy: 34% | 0.11 |
TESTED questionnaire (n = 12) | |||
Vocalizations | Presence of vocalization. | easy: 83% | 0.83 |
normal: 17% | |||
Spontaneous blinking | TESTED questionnaire (n = 8) | ||
Presence of natural blinking. | easy: 50% | 0.57 | |
Tonic–clonic seizures | TESTED questionnaire (n = 16) | ||
Absence of tonic seizure. | easy: 69% | 0.69 | |
normal: 31% | |||
Muscle tone | TESTED questionnaire (n = 7) | ||
Loss of muscle tone (floppy ears and relaxed jaws). | easy: 100% | 1 |
ABMs | References | Study Environment | Animal Category | Stunning Method | Restraining Method |
---|---|---|---|---|---|
Breathing | [30] | F | L | N-PCB | NO |
[20] | E | L | AN | NO | |
[31] | ES | L | HO | VB | |
[32] | S | L | HO | G | |
[33] | F | S | PCB | NO | |
[34] | ES | L | HO | NO | |
[9] | E | L | HO | NO | |
[35] | E | L | HO | NO | |
Posture | [33] | F | S | PCB | NO |
Tonic–clonic seizure | [30] | F | L | N-PCB | NO |
[31] | ES | L | HO | VB | |
[32] | S | L | HO | G | |
[34] | ES | L | HO | NO | |
[9] | E | L | HO | NO | |
[35] | E | L | HO | NO | |
Corneal reflex | [30] | F | L | N-PCB | NO |
[31] | ES | L | HO | VB | |
[32] | S | L | HO | G | |
[33] | F | S | PCB | NO | |
[34] | ES | L | HO | NO | |
[9] | E | L | HO | NO | |
[35] | E | L | HO | NO | |
Palpebral reflex | [30] | F | L | N-PCB | NO |
[20] | E | L | AN | NO | |
[33] | F | S | PCB | NO | |
Spontaneous blinking | [31] | ES | L | HO | VB |
Muscle tone | [32] | S | L | HO | G |
[33] | F | S | PCB | NO | |
Eye movements | [32] | S | L | HO | G |
[33] | E | S | PCB | NO |
ABMs | References | Time | Position | Distance | Access | Equipment | Observational Constraint |
---|---|---|---|---|---|---|---|
Breathing | [30] | Un | Un | Un | No | Un | Un |
[20] | Un | Un | Un | No | Yes | Un | |
[31] | 4–8 s 1 | Un | Un | No | Yes | Un | |
[32] | 4–8 s 1 | Un | Un | No | No | Un | |
[33] | Un | Un | Un | No | Un | Un | |
[34] | 4–8 s 1 | Un | Un | No | No | Un | |
[9] | Un | Un | Un | No | Yes | Un | |
[35] | Un | Un | Un | No | Yes | Un | |
Posture | [33] | Un | Un | Un | No | Un | Un |
Tonic–clonic seizure | [30] | Un | Un | Un | No | Un | Un |
[31] | Un | Un | Un | No | Yes | Un | |
[32] | Un | Un | Un | No | Un | Un | |
[34] | Un | Un | Un | No | Un | Un | |
[9] | Un | Un | Un | No | Yes | Un | |
[35] | Un | Un | Un | No | Yes | Un | |
Corneal reflex | [30] | Un | Un | Un | Yes | No | Un |
[31] | Un | Un | Un | Yes | Yes | Un | |
[32] | Un | Un | Un | Yes | No | Un | |
[33] | Un | Un | Un | Yes | No | Un | |
[34] | Un | Un | Un | Yes | No | Un | |
[9] | Un | Un | Un | Yes | Yes | Un | |
[35] | Un | Un | Un | Yes | Yes | Un | |
Palpebral reflex | [30] | Un | Un | Un | Yes | No | Un |
[20] | Un | Un | Un | Yes | Yes | Un | |
[33] | Un | Un | Un | Yes | No | Un | |
Spontaneous blinking | [31] | Un | Un | Un | No | Yes | Un |
Muscle tone | [32] | Un | Un | Un | No | Un | Un |
[33] | Un | Un | Un | No | Un | Un | |
Eye movements | [32] | Un | Un | Un | No | Un | Un |
[33] | Un | Un | Un | No | Un | Un |
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Comin, M.; Barbieri, S.; Minero, M.; Dalla Costa, E. The Feasibility of Animal-Based Indicators of Consciousness and Unconsciousness for Stunning in Sheep: A Systematic Review. Animals 2023, 13, 1395. https://doi.org/10.3390/ani13081395
Comin M, Barbieri S, Minero M, Dalla Costa E. The Feasibility of Animal-Based Indicators of Consciousness and Unconsciousness for Stunning in Sheep: A Systematic Review. Animals. 2023; 13(8):1395. https://doi.org/10.3390/ani13081395
Chicago/Turabian StyleComin, Marta, Sara Barbieri, Michela Minero, and Emanuela Dalla Costa. 2023. "The Feasibility of Animal-Based Indicators of Consciousness and Unconsciousness for Stunning in Sheep: A Systematic Review" Animals 13, no. 8: 1395. https://doi.org/10.3390/ani13081395
APA StyleComin, M., Barbieri, S., Minero, M., & Dalla Costa, E. (2023). The Feasibility of Animal-Based Indicators of Consciousness and Unconsciousness for Stunning in Sheep: A Systematic Review. Animals, 13(8), 1395. https://doi.org/10.3390/ani13081395