Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Horses and Sample Preparation
2.2. FT Raman Spectroscopy and Data Analysis
2.3. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Data Points | ||||
---|---|---|---|---|
Before | After Exercise | After 30 min | After 60 min | |
Rectal temperature (°C) | 36.98 ± 0.34 | 37.94 ± 0.43 | 37.56 ± 0.28 | 37.22 ± 0.19 |
Heart rate (bpm) | 39.60 ± 2.60 | 199.00 ± 21.90 | 55.20 ± 3.34 | 40.80 ± 3.03 |
Lactic acid (mmol/L) | 1.11 ± 0.07 | 4.06 ± 0.34 | 2.10 ± 0.66 | 1.23 ± 0.18 |
Glucose (mg/dL) | 74.80 ± 6.76 | 55.60 ± 8.90 | 76.40 ± 4.33 | 76.20 ± 6.26 |
Sub-Band ID | Center Frequency (cm−1) | Metabolic Biomarker | Vibrational Mode | References |
---|---|---|---|---|
A | 1312 | Lipids | τ(CH3CH2) | [31,33,34] |
B | 1336 | Tryptophan | γ(CH2) | [35,36] |
Sub-Band ID | Center Frequency (cm−1) | Metabolic Biomarker | Vibrational Mode | References |
---|---|---|---|---|
1 | 1393 | Leucine | γ(CH2), δ(CCH) | [32,36,37] |
2 | 1413 | Glycine | s(COO−) | [36,38] |
3 | 1437 | Isoleucine | Cγ—asym rock; C δsym bend | [32,37,38,39] |
4 | 1455 | Lactic Acid, Lipids | CH3 asym bend; CH3 rock | [40,41,42,43,44,45] |
5 | 1470 | Tripeptide | σ(CH2); γ(NH3+) | [36] |
6 | 1489 | Adenosine | σ(CH2) | [46] |
7 | 1516 | Beta Carotene | C-C sym; s(C=C) | [47] |
1300–1360 cm−1 Range | ||||
---|---|---|---|---|
Sub-band A (Collagen/lipids) | ||||
Data points | ||||
Before | After exercise | After 30 min | After 60 min | |
Mean ± SD | 16.92 ± 3.26 | 47.37 ± 9.87 | 25.81 ± 14.35 | 10.46 ± 3.21 |
Before | 0.0001 | 0.01 | NS | |
After exercise | 0.0001 | 0.001 | 0.0001 | |
After 30 min | 0.01 | 0.001 | 0.001 | |
After 60 min | NS | 0.0001 | 0.001 | |
Sub-band B (Tryptophan) | ||||
Data points | ||||
Before | After exercise | After 30 min | After 60 min | |
Mean ± SD | 36.77 ± 2.86 | 43.16 ± 5.40 | 52.98 ± 6.63 | 24.74 ± 6.50 |
Before | NS | 0.001 | 0.01 | |
After exercise | NS | 0.01 | 0.0001 | |
After 30 min | 0.001 | 0.01 | 0.0001 | |
After 60 min | 0.01 | 0.0001 | 0.0001 |
1385–1520 cm−1 Range | |||||
---|---|---|---|---|---|
Data Points | |||||
Before | After Exercise | After 30 min | After 60 min | ||
Sub-band 1 (Leucine) | Mean ± SD | 96.38 ± 18.61 | 154.70 ± 27.84 | 94.06 ± 3.56 | 74.43 ± 0.71 |
Before | 0.0001 | NS | NS | ||
After exercise | 0.0001 | 0.0001 | 0.0001 | ||
After 30 min | NS | 0.0001 | NS | ||
After 60 min | NS | 0.0001 | NS | ||
Sub-band 2 (Glycine) | Mean ± SD | 63.68 ± 12.28 | 29.26 ± 5.02 | 58.90 ± 4.24 | 59.46 ± 14.94 |
Before | 0.001 | NS | NS | ||
After exercise | 0.001 | 0.01 | 0.001 | ||
After 30 min | NS | 0.01 | NS | ||
After 60 min | NS | 0.001 | NS | ||
Sub-band 3 (Isoleucine) | Mean ± SD | 171.80 ± 43.51 | 81.80 ± 15.08 | 190.50 ± 29.00 | 200.70 ± 60.85 |
Before | 0.01 | NS | NS | ||
After exercise | 0.01 | 0.001 | 0.001 | ||
After 30 min | NS | 0.001 | NS | ||
After 60 min | NS | 0.001 | NS | ||
Sub-band 4 (Lactic acid) | Mean ± SD | 115.10 ± 29.62 | 184.80 ± 27.36 | 123.10 ± 16.79 | 85.34 ± 9.97 |
Before | 0.001 | NS | NS | ||
After exercise | 0.001 | 0.01 | 0.001 | ||
After 30 min | NS | 0.01 | NS | ||
After 60 min | NS | 0.001 | NS | ||
Sub-band 5 (Tripeptide) | Mean ± SD | 29.48 ± 9.15 | 44.70 ± 14.39 | 34.16 ± 7.98 | 31.72 ± 8.91 |
Before | 0.001 | NS | NS | ||
After exercise | 0.001 | 0.01 | 0.001 | ||
After 30 min | NS | 0.01 | NS | ||
After 60 min | NS | 0.001 | NS | ||
Sub-band 6 (Adenosine) | Mean ± SD | 73.93 ± 8.36 | 58.24 ± 5.66 | 57.16 ± 8.02 | 61.68 ± 8.49 |
Before | 0.01 | 0.01 | NS | ||
After exercise | 0.01 | NS | NS | ||
After 30 min | 0.01 | NS | NS | ||
After 60 min | NS | NS | NS | ||
Sub-band 7 (Beta carotene) | Mean ± SD | 29.61 ± 9.08 | 23.35 ± 6.48 | 20.28 ± 5.91 | 35.05 ± 15.42 |
Before | NS | NS | NS | ||
After exercise | NS | NS | 0.05 | ||
After 30 min | NS | NS | 0.01 | ||
After 60 min | NS | 0.05 | 0.01 |
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Acri, G.; Testagrossa, B.; Piccione, G.; Arfuso, F.; Giudice, E.; Giannetto, C. Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy. Animals 2023, 13, 2201. https://doi.org/10.3390/ani13132201
Acri G, Testagrossa B, Piccione G, Arfuso F, Giudice E, Giannetto C. Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy. Animals. 2023; 13(13):2201. https://doi.org/10.3390/ani13132201
Chicago/Turabian StyleAcri, Giuseppe, Barbara Testagrossa, Giuseppe Piccione, Francesca Arfuso, Elisabetta Giudice, and Claudia Giannetto. 2023. "Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy" Animals 13, no. 13: 2201. https://doi.org/10.3390/ani13132201