Seasonal and Nutritional Fluctuations in the mRNA Levels of the Short Form of the Leptin Receptor (LRa) in the Hypothalamus and Anterior Pituitary in Resistin-Treated Sheep
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Experimental Design
2.3. Animal Treatments
2.3.1. Experiment 1. The Effects of Photoperiod and RbresistinTreatment on the Expression of LRa in Selected Brain Areas and Hormone Concentrations
2.3.2. Experiment 2. The Effect of BW and Rbresistin Treatment on the Expression of LRa in Selected Brain Areas and Hormone Concentrations
2.4. Hormone Assays
2.5. Statistics
2.5.1. Statistical Analysis
2.5.2. Molecular Analysis
2.5.3. Molecular Data Analysis
3. Results
3.1. The Effects of Photoperiod and Rbresistin Treatment on the Expression of LRa in Selected Brain Areas and Hormone Concentrations
3.1.1. Expression of mRNA LRa in Selected Brain Areas
3.1.2. Estradiol Concentration
3.1.3. Resistin Concentrations
3.1.4. Leptin Concentrations
3.2. The Effect of BW and Rbresistin Treatment on the Expression of LRa in Selected Brain Areas and Hormone Concentrations
3.2.1. Expression of mRNA LRa in Selected Brain Areas
3.2.2. Resistin Concentrations
3.2.3. Leptin Concentrations
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | PrimerSequence (5′–3′) | Probe Sequence (5′–3′) | Amplicon Size | Gen Bank Accession Number |
---|---|---|---|---|
CPH | CGGCTCCCAGTTCTTCATCA | FAM-CGTTCCGACTCCGC-MGB | 64 bp | D14074 |
ACTACGTGCTTCCCATCCAAA | ||||
LRa | TCAAAGTATGTCCGTTCTCTTCTG | FAM-TGTTTTGGGAAGATGTTC-MGB | 131 bp | NM_001009763.1 |
TCTTATTGCTTGGAACATTGTCA |
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Biernat, W.; Szczęsna, M.; Kirsz, K.; Zieba, D.A. Seasonal and Nutritional Fluctuations in the mRNA Levels of the Short Form of the Leptin Receptor (LRa) in the Hypothalamus and Anterior Pituitary in Resistin-Treated Sheep. Animals 2021, 11, 2451. https://doi.org/10.3390/ani11082451
Biernat W, Szczęsna M, Kirsz K, Zieba DA. Seasonal and Nutritional Fluctuations in the mRNA Levels of the Short Form of the Leptin Receptor (LRa) in the Hypothalamus and Anterior Pituitary in Resistin-Treated Sheep. Animals. 2021; 11(8):2451. https://doi.org/10.3390/ani11082451
Chicago/Turabian StyleBiernat, Weronika, Malgorzata Szczęsna, Katarzyna Kirsz, and Dorota Anna Zieba. 2021. "Seasonal and Nutritional Fluctuations in the mRNA Levels of the Short Form of the Leptin Receptor (LRa) in the Hypothalamus and Anterior Pituitary in Resistin-Treated Sheep" Animals 11, no. 8: 2451. https://doi.org/10.3390/ani11082451
APA StyleBiernat, W., Szczęsna, M., Kirsz, K., & Zieba, D. A. (2021). Seasonal and Nutritional Fluctuations in the mRNA Levels of the Short Form of the Leptin Receptor (LRa) in the Hypothalamus and Anterior Pituitary in Resistin-Treated Sheep. Animals, 11(8), 2451. https://doi.org/10.3390/ani11082451