The Apelinergic System Immuno-Detection in the Abomasum and Duodenum of Sheep Grazing on Semi-Natural Pasture
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal Recruiting and Sample Collection
2.2. Morphological Staining and Immunohistochemistry
2.3. Double-Label Immunohistochemistry
2.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Organs | Searched Molecules | Immunoreactive Structures | M × F | E × p | M × D |
---|---|---|---|---|---|
Abomasum | APLN | Lining epithelium | 1.15 | 1.35 | 1 |
Fundic glands | 1.98 | 2.13 | 1.18 | ||
APLNR | Lining epithelium | 0.85 | 0.87 | 0.48 | |
Fundic glands | 1.18 | 1.2 | 0.31 | ||
Duodenum | APLNR | Lining epithelium | 1.87 | 1.79 | 1.03 |
Intestinal crypts | 1.5 | 1.66 | 0.84 | ||
Neuroendocrine cells | 1.7 | 2.33 | 2.7 |
Organs | Searched Molecules | Immunoreactive Structures | Kruskal–WallisTest | Wilcoxon Rank Sum Test | ||
---|---|---|---|---|---|---|
M × F vs. E × p | M × F vs. M × D | E × p vs. M × D | ||||
Abomasum | APLN | Lining epithelium | 0.1567 | 0.41980 | 0.41980 | 0.22803 |
Fundic glands | 1.439 × 10−7 | 0.0702 | 7.92 × 10−6 | 7.92 × 10−6 | ||
APLNR | Lining epithelium | 0.000352 | 0.8329 | 0.00171 | 0.00171 | |
Fundic glands | 3.129 × 10−7 | 0.7981 | 8.277 × 10−6 | 8.277 × 10−6 | ||
Duodenum | APLNR | Lining epithelium | 2.827 × 10−7 | 0.4076 | 8.517 × 10−6 | 8.517 × 10−6 |
Intestinal crypts | 1.716 × 10−6 | 0.2282 | 1.453 × 10−4 | 8.829 × 10−6 | ||
Neuroendocrine cells | 0.002526 | 0.0601 | 0.0080 | 0.0601 |
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Palmioli, E.; Dall’Aglio, C.; Bellesi, M.; Tardella, F.M.; Moscatelli, S.; Scocco, P.; Mercati, F. The Apelinergic System Immuno-Detection in the Abomasum and Duodenum of Sheep Grazing on Semi-Natural Pasture. Animals 2021, 11, 3173. https://doi.org/10.3390/ani11113173
Palmioli E, Dall’Aglio C, Bellesi M, Tardella FM, Moscatelli S, Scocco P, Mercati F. The Apelinergic System Immuno-Detection in the Abomasum and Duodenum of Sheep Grazing on Semi-Natural Pasture. Animals. 2021; 11(11):3173. https://doi.org/10.3390/ani11113173
Chicago/Turabian StylePalmioli, Elisa, Cecilia Dall’Aglio, Michele Bellesi, Federico Maria Tardella, Sara Moscatelli, Paola Scocco, and Francesca Mercati. 2021. "The Apelinergic System Immuno-Detection in the Abomasum and Duodenum of Sheep Grazing on Semi-Natural Pasture" Animals 11, no. 11: 3173. https://doi.org/10.3390/ani11113173