Integrated Analysis of miRNA-mRNA Network Reveals Different Regulatory Patterns in the Endometrium of Meishan and Duroc Sows during Mid-Late Gestation
1
Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, MOA Laboratory of Animal Genetics and Breeding, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
2
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
*
Author to whom correspondence should be addressed.
†
These authors are contributed equally.
Animals 2020, 10(3), 420; https://doi.org/10.3390/ani10030420
Received: 19 February 2020
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Revised: 29 February 2020
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Accepted: 1 March 2020
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Published: 3 March 2020
(This article belongs to the Collection Genomics, Transcriptomics, and Computational Biology for Biodiversity Studies and Quality-Related Traits Selection in Livestock)
Simple Summary
Meishan pigs have a lower fetal loss rate during mid-late gestation compared to Duroc pigs. Differentially expressed mRNAs and miRNAs detected in endometrial tissue from Meishan and Duroc sows at mid-late gestation are involved in regulating hormone and oxygen levels, blood vessel development, and developmental processes affecting reproduction. In addition, ssc-miR-503 and ssc-miR-671-5p were shown to target the EGF and ESR1 genes, respectively. These findings provided an important resource for studying embryonic mortality during mid-late gestation in pigs.
Embryo loss is a major factor affecting profitability in the pig industry. Embryonic mortality occurs during peri-implantation and mid-late gestation in pigs. Previous investigations have shown that the embryo loss rate in Meishan pigs is significantly lower than in commercial breeds. Most studies have focused on embryonic mortality during early gestation, but little is known about losses during mid-late gestation. In this study, we performed a transcriptome analysis of endometrial tissue in mid-late gestation sows (gestation days 49 and 72) sampled from two breeds (Meishan (MS) and Duroc (DU)) that have different embryo loss rates. We identified 411, 1113, 697, and 327 differentially expressed genes, and 14, 36, 57, and 43 differentially expressed miRNAs in four comparisons (DU49 vs. DU72, DU49 vs. MS49, DU72 vs. MS72, and MS49 vs. MS72), respectively. Subsequently; seven differentially expressed mRNAs and miRNAs were validated using qPCR. Functional analysis suggested the differentially expressed genes and miRNAs target genes mainly involved in regulation of hormone levels, blood vessel development, developmental process involved in reproduction, embryonic placenta development, and the immune system. A network analysis of potential miRNA-gene interactions revealed that differentially expressed miRNAs in Meishan pigs are involved in the response to estradiol and oxygen levels, and affect angiogenesis and blood vessel development. The binding site on ssc-miR-503 for epidermal growth factor (EGF) and the binding site on ssc-miR-671-5p for estrogen receptor α (ESR1) were identified using a dual luciferase assay. The results of this study will enable further exploration of miRNA-mRNA interactions important in pig pregnancy and will help to uncover molecular mechanisms affecting embryonic mortality in pigs during mid-late gestation.
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Keywords:
RNA-seq; miRNA-mRNA interaction; endometrium; Meishan and Duroc sows
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MDPI and ACS Style
Yang, K.; Wang, J.; Wang, K.; Luo, Y.; Tang, Q.; Liu, X.; Fang, M. Integrated Analysis of miRNA-mRNA Network Reveals Different Regulatory Patterns in the Endometrium of Meishan and Duroc Sows during Mid-Late Gestation. Animals 2020, 10, 420. https://doi.org/10.3390/ani10030420
AMA Style
Yang K, Wang J, Wang K, Luo Y, Tang Q, Liu X, Fang M. Integrated Analysis of miRNA-mRNA Network Reveals Different Regulatory Patterns in the Endometrium of Meishan and Duroc Sows during Mid-Late Gestation. Animals. 2020; 10(3):420. https://doi.org/10.3390/ani10030420
Chicago/Turabian StyleYang, Kaijie, Jue Wang, Kejun Wang, Yabiao Luo, Qiguo Tang, Ximing Liu, and Meiying Fang. 2020. "Integrated Analysis of miRNA-mRNA Network Reveals Different Regulatory Patterns in the Endometrium of Meishan and Duroc Sows during Mid-Late Gestation" Animals 10, no. 3: 420. https://doi.org/10.3390/ani10030420
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