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Open AccessArticle

Antibacterial and Hemolytic Activity of Crotalus Triseriatus and Crotalus Ravus Venom

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Universidad Autónoma del Estado de Hidalgo, Área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuarias, Rancho Universitario Av. Universidad km 1, EX-Hda de Aquetzalpa, Tulancingo, Hidalgo 43600, México
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Universidad Autónoma del Estado de Hidalgo, Área Académica de Biología, Instituto de Ciencias Básicas e Ingeniería. Carretera Pachuca-Tulancingo S/N Int. 22 Colonia Carboneras, Mineral de la Reforma, Hidalgo 42180, México
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Facultad de Medicina Veterinaria y Zootecnia Universidad Autónoma del Estado de México, El Cerrillo Piedras Blancas, Toluca 50295, México
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Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID SAI-INIFAP), Carretera Federal Cuernavaca-Cuautla No. 8534/Col. Progreso, Jiutepec 62550, Morelos, México
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Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Isla Teja s/n, Casilla 567, Valdivia 5090000, Chile
*
Author to whom correspondence should be addressed.
Animals 2020, 10(2), 281; https://doi.org/10.3390/ani10020281 (registering DOI)
Received: 14 January 2020 / Revised: 7 February 2020 / Accepted: 7 February 2020 / Published: 11 February 2020
(This article belongs to the Special Issue Veterinary Microbiology & Parasitology)
Rattlesnakes have venoms with a complex toxin mixture comprised of polypeptides and proteins. Previous studies have shown that some of these polypeptides are of high value for the development of new medical treatments. The aim of the present study is to evaluate, in vitro, the antibacterial and hemolytic activity of Crotalus triseriatus and Crotalus ravus venoms. A direct field search was conducted to obtain Crotalus triseriatus and Crotalus ravus venom samples. These were evaluated to determine their antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa through the techniques of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Hemolytic activity was also determined. Antibacterial activity was determined for treatments (Crotalus triseriatus 2) CT2 and (Crotalus ravus 3) CR3, obtaining a Minimum Inhibitory Concentration of 50 µg/mL and a Minimum Bactericidal Concentration of 100 µg/mL against Pseudomonas aeruginosa. CT1 (Crotalus triseriatus 1), CT2, and CR3 presented hemolytic activity; on the other hand, Crotalus ravus 4 (CR4) did not show hemolytic activity. The results of the present study indicate for the first time that Crotalus triseriatus and Crotalus ravus venoms contain some bioactive compounds with bactericidal activity against Pseudomonas aeruginosa which could be used as alternative treatment in diseases caused by this pathogenic bacterium.
Keywords: Crotalus ravus; Crotalus triseriatus; venom; antibacterial activity; Pseudomonas aeruginosa; hemolytic activity Crotalus ravus; Crotalus triseriatus; venom; antibacterial activity; Pseudomonas aeruginosa; hemolytic activity
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MDPI and ACS Style

Zaragoza-Bastida, A.; Flores-Aguilar, S.C.; Aguilar-Castro, L.M.; Morales-Ubaldo, A.L.; Valladares-Carranza, B.; Rangel-López, L.; Olmedo-Juárez, A.; Rosenfeld-Miranda, C.E.; Rivero-Pérez, N. Antibacterial and Hemolytic Activity of Crotalus Triseriatus and Crotalus Ravus Venom. Animals 2020, 10, 281.

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