Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Animals
2.3. Donor Cell Isolation, Ovary Collection, and Oocyte Maturation
2.4. SCNT
2.5. Embryo Transfer
2.6. Analysis of Recipient Pregnancy and Delivery of Cloned Piglets
2.7. Measurement of Growth Performance
2.8. Evaluation of Semen Quality
2.9. Measurement of Reproductive Performance
2.10. Progeny Test
2.11. Statistical Analysis
3. Results
3.1. Donor Boar Selection
3.2. Production of Clones
3.3. Growth Performance
3.4. Semen Quality
3.5. Breeding Test
3.6. Progeny Test
3.7. Coefficient of Variation Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Parameters | Group | Average | |||
---|---|---|---|---|---|
Boar 1 | Boar 2 | Boar 3 | Boar 4 | ||
No. of recipient sows | 50 | 148 | 27 | 25 | 62.50 |
No. of cloned embryo | 11,068 | 36,483 | 5771 | 5739 | 14,765 |
Pregnancy rate (%) | 76.00 | 72.97 | 77.78 | 76.00 | 74.40 |
Farrowing rate (%) | 48.00 | 45.95 | 33.33 | 44.00 | 44.80 |
No. of total piglets | 91 | 197 | 31 | 48 | 92 |
No. of born-alive piglets | 59 | 155 | 23 | 38 | 69 |
No. of weaned piglets | 33 | 56 | 8 | 14 | 28 |
Average litter size | 3.79 ± 0.37 b | 2.89 ± 0.18 a | 3.44 ± 0.50 a,b | 4.36 ± 0.80 b | 3.28 ± 0.17 |
Developmental rate of cloned embryos (%) | 0.86 a | 0.58 a | 0.56 a | 0.98 a | 0.67 |
Parameters | Clone (n = 19) | Nonclone (n = 28) | p Values |
---|---|---|---|
No. of ejaculates | 507 | 563 | |
Semen volume (mL) | 232.95 ± 12.17 | 237.47 ± 9.91 | 0.774 |
Sperm motility | 0.71 ± 0.02 | 0.71 ± 0.02 | 0.128 |
Sperm concentration (108/mL) | 2.77 ± 0.18 | 2.58 ± 0.47 | 0.544 |
Percentage of abnormal sperm (%) | 8.42 ± 0.48 | 8.48 ± 0.58 | 0.950 |
Parameters | Clone (n = 21) | Nonclone (n = 25) | p Values |
---|---|---|---|
No. of sows | 1380 | 1437 | |
Total piglets/litter | 9.90 ± 0.07 | 10.19 ± 0.16 | 0.117 |
Born-alive piglets/litter | 8.80 ± 0.08 | 9.14 ± 0.17 | 0.080 |
Weak piglets/litter | 0.46 ± 0.03 | 0.41 ± 0.04 | 0.741 |
Malformed piglets/litter | 0.47 ± 0.05 | 0.38 ± 0.03 | 0.072 |
Mummified piglets/litter | 0.51 ± 0.05 | 0.54 ± 0.05 | 0.843 |
Still-born piglets/litter | 0.59 ± 0.05 | 0.51 ± 0.08 | 0.035 |
Weaned piglets/litter | 7.83 ± 0.06 | 7.93 ± 0.10 | 0.225 |
Litter weight of piglets (kg)/litter | 14.27 ± 0.15 | 14.72 ± 0.20 | 0.158 |
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Shi, J.; Tan, B.; Luo, L.; Li, Z.; Hong, L.; Yang, J.; Cai, G.; Zheng, E.; Wu, Z.; Gu, T. Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars. Animals 2020, 10, 2053. https://doi.org/10.3390/ani10112053
Shi J, Tan B, Luo L, Li Z, Hong L, Yang J, Cai G, Zheng E, Wu Z, Gu T. Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars. Animals. 2020; 10(11):2053. https://doi.org/10.3390/ani10112053
Chicago/Turabian StyleShi, Junsong, Baohua Tan, Lvhua Luo, Zicong Li, Linjun Hong, Jie Yang, Gengyuan Cai, Enqin Zheng, Zhenfang Wu, and Ting Gu. 2020. "Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars" Animals 10, no. 11: 2053. https://doi.org/10.3390/ani10112053
APA StyleShi, J., Tan, B., Luo, L., Li, Z., Hong, L., Yang, J., Cai, G., Zheng, E., Wu, Z., & Gu, T. (2020). Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars. Animals, 10(11), 2053. https://doi.org/10.3390/ani10112053