Identification of Novel lncRNAs Differentially Expressed in Placentas of Chinese Ningqiang Pony and Yili Horse Breeds
Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
International Livestock Research Institute (ILRI), Nairobi 00100, Kenya
Authors to whom correspondence should be addressed.
These authors contribute equally to this work.
Received: 11 November 2019 / Revised: 30 December 2019 / Accepted: 9 January 2020 / Published: 11 January 2020
The Ningqiang pony (NQ) is a famous breed with an average adult height at the withers (the ridge between horse’s shoulder blades) of less than 106 cm, but the genetic mechanism responsible for its small stature remains unclear. Research on Chinese pony breeds is a topic of theoretical and practical importance. Long non-coding RNA (lncRNA) has been shown to regulate many biological functions, including growth. Our study, for the first time, reports the lncRNAs transcribed in horse placentas. By comparing 3011 transcripts coding 1464 lncRNAs from NQ ponies and Yili horses (YL) with distinct body sizes, six (TBX3 (T-box3), CACNA1F (L-type voltage-dependent calcium channel a1F), EDN3 (endothelin-3), KAT5 (histone acetyltransferase KAT5), ZNF281 (zinc finger protein 281), TMED2 (transmembrane emp24 domain), and TGFB1 (transforming growth factor beta 1)) out of the 233 genes targeted by differentially expressed lncRNAs were identified as being involved in limb development, skeletal myoblast differentiation, and embryo development. These results provide new insights into the genetic architecture of horse body size.