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Article

Impact of Full Vaccination with mRNA BNT162b2 on SARS-CoV-2 Infection: Genomic and Subgenomic Viral RNAs Detection in Nasopharyngeal Swab and Saliva of Health Care Workers

1
Department of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy
2
Laboratory of Experimental Animal Models, Division of Comparative Biomedical Sciences, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy
3
Department of Diagnostics and Public Health, University of Verona, 37129 Verona, Italy
*
Authors to whom correspondence should be addressed.
Academic Editor: Deepak Shukla
Microorganisms 2021, 9(8), 1738; https://doi.org/10.3390/microorganisms9081738
Received: 20 July 2021 / Revised: 2 August 2021 / Accepted: 12 August 2021 / Published: 14 August 2021
(This article belongs to the Special Issue COVID-19: Focusing on Epidemiologic, Virologic, and Clinical Studies)
SARS-CoV-2 infection was monitored in 1898 health care workers (HCWs) after receiving full vaccination with BNT162b2. Untill 30 June 2021, 10 HCWs tested positive for SARS-CoV-2 using real time RT-PCR, resulting in a 4-month cumulative incidence of 0.005%. The infection was mildly symptomatic in six (60%) and asymptomatic in four (40%) individuals. Among the infected HCWs, eight consenting individuals provided paired NPS and saliva during the course of infection, for the purpose of the analysis performed in the present study. Genomic and subgenomic viral RNAs were investigated using real-time RT-PCR in both biological specimens. The temporal profile of viral load was measured using ddPCR. Viral mutations were also analysed. Subgenomic viral RNA was detected in 8/8 (100%) NPS and in 6/8 (75%) saliva specimens at the baseline. The expression of subgenomic RNA was observed for up to 7 days in 3/8 (38%) symptomatic cases. Moreover, concordance was observed between NPS and saliva in the detection of viral mutations, and both N501Y and 69/70del (associated with the B.1.1.7 variant) were detected in the majority 6/8 (75%) of subjects, while the K417T mutation (associated with the P.1-type variants) was detected in 2/8 (25%) individuals. Overall, our findings report a low frequency of infected HCWs after full vaccination. It is, therefore, important to monitor the vaccinees in order to identify asymptomatic infected individuals. Saliva can be a surrogate for SARS-CoV-2 surveillance, particularly in social settings such as hospitals. View Full-Text
Keywords: SARS-CoV-2; coronavirus; subgenomic; saliva; vaccine; BNT162b2; transmission; real time RT-PCR; droplet digital PCR SARS-CoV-2; coronavirus; subgenomic; saliva; vaccine; BNT162b2; transmission; real time RT-PCR; droplet digital PCR
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MDPI and ACS Style

Deiana, M.; Mori, A.; Piubelli, C.; Perandin, F.; Treggiari, D.; Martini, D.; Chesini, F.; Angheben, A.; Bonfante, F.; Terregino, C.; Bisoffi, Z.; Pomari, E. Impact of Full Vaccination with mRNA BNT162b2 on SARS-CoV-2 Infection: Genomic and Subgenomic Viral RNAs Detection in Nasopharyngeal Swab and Saliva of Health Care Workers. Microorganisms 2021, 9, 1738. https://doi.org/10.3390/microorganisms9081738

AMA Style

Deiana M, Mori A, Piubelli C, Perandin F, Treggiari D, Martini D, Chesini F, Angheben A, Bonfante F, Terregino C, Bisoffi Z, Pomari E. Impact of Full Vaccination with mRNA BNT162b2 on SARS-CoV-2 Infection: Genomic and Subgenomic Viral RNAs Detection in Nasopharyngeal Swab and Saliva of Health Care Workers. Microorganisms. 2021; 9(8):1738. https://doi.org/10.3390/microorganisms9081738

Chicago/Turabian Style

Deiana, Michela, Antonio Mori, Chiara Piubelli, Francesca Perandin, Davide Treggiari, Davide Martini, Fabio Chesini, Andrea Angheben, Francesco Bonfante, Calogero Terregino, Zeno Bisoffi, and Elena Pomari. 2021. "Impact of Full Vaccination with mRNA BNT162b2 on SARS-CoV-2 Infection: Genomic and Subgenomic Viral RNAs Detection in Nasopharyngeal Swab and Saliva of Health Care Workers" Microorganisms 9, no. 8: 1738. https://doi.org/10.3390/microorganisms9081738

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