Next Article in Journal
Inoculation with Mycorrhizal Fungi and Irrigation Management Shape the Bacterial and Fungal Communities and Networks in Vineyard Soils
Next Article in Special Issue
Development and Clinical Evaluation of Serum and Urine-Based Lateral Flow Tests for Diagnosis of Human Visceral Leishmaniasis
Previous Article in Journal / Special Issue
Malnutrition Aggravates Alterations Observed in the Gut Structure and Immune Response of Mice Infected with Leishmania infantum
Article

MPLA and AddaVax® Adjuvants Fail to Promote Intramuscular LaAg Vaccine Protectiveness against Experimental Cutaneous Leishmaniasis

1
Laboratório de Imunofarmacologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
2
Laboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil
3
Grupo de Imunologia e Vacinologia, Instituto de Microbiologia Paulo de Goés, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
4
Núcleo de Doenças Infecciosas/Núcleo de Biotecnologia-Universidade Federal do Espírito Santo, Vitória 29075-910, Brazil
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Helena Castro
Microorganisms 2021, 9(6), 1272; https://doi.org/10.3390/microorganisms9061272
Received: 30 January 2021 / Accepted: 4 March 2021 / Published: 11 June 2021
(This article belongs to the Special Issue Leishmania and Leishmaniasis)
There is so far no vaccine approved for human leishmaniasis, mainly because of the lack of appropriate adjuvants. This study aimed to evaluate in mice the capacity of a mixture of monophosphoryl lipid A (MPLA) and AddaVax® adjuvants in enhancing the efficacy of a Leishvacin®-like vaccine comprised of Leishmania amazonensis whole antigens (LaAg). For that, mice were immunized with LaAg plus MPLA/AddaVax® by the intramuscular route (i.m.) prior to challenge with 2 × 105 and 2 × 106 living parasites. Immunization with LaAg alone reduced the lesion growth of the 2 × 105-challenged mice only in the peak of infection, but that was not accompanied by reduced parasite load, and thus not considered protective. Mice given a 2 × 106 -challenge were not protected by LaAg. The association of LaAg with MPLA/AddaVax® was able to enhance the cutaneous hypersensitivity response compared with LaAg alone. Despite this, there was no difference in proliferative cell response to antigen ex vivo. Moreover, regardless of the parasite challenge, association of LaAg with MPL/AddaVax® did not significantly enhance protection in comparison with LaAg alone. This work demonstrated that MPL/AddaVax® is not effective in improving the efficacy of i.m. LaAg vaccine against cutaneous leishmaniasis. View Full-Text
Keywords: Leishmania amazonensis; LaAg vaccine; adjuvants; MPLA; AddaVax®; intramuscular; immunization; C57BL/6 Leishmania amazonensis; LaAg vaccine; adjuvants; MPLA; AddaVax®; intramuscular; immunization; C57BL/6
Show Figures

Figure 1

MDPI and ACS Style

Oliveira-Maciel, D.; dos-Santos, J.S.; Oliveira-Silva, G.; Mello, M.F.d.; da Fonseca-Martins, A.M.; Carneiro, M.P.D.; Ramos, T.D.; Firmino-Cruz, L.; Gomes, D.C.O.; Rossi-Bergmann, B.; de Matos Guedes, H.L. MPLA and AddaVax® Adjuvants Fail to Promote Intramuscular LaAg Vaccine Protectiveness against Experimental Cutaneous Leishmaniasis. Microorganisms 2021, 9, 1272. https://doi.org/10.3390/microorganisms9061272

AMA Style

Oliveira-Maciel D, dos-Santos JS, Oliveira-Silva G, Mello MFd, da Fonseca-Martins AM, Carneiro MPD, Ramos TD, Firmino-Cruz L, Gomes DCO, Rossi-Bergmann B, de Matos Guedes HL. MPLA and AddaVax® Adjuvants Fail to Promote Intramuscular LaAg Vaccine Protectiveness against Experimental Cutaneous Leishmaniasis. Microorganisms. 2021; 9(6):1272. https://doi.org/10.3390/microorganisms9061272

Chicago/Turabian Style

Oliveira-Maciel, Diogo; dos-Santos, Júlio S.; Oliveira-Silva, Gabriel; Mello, Mirian F.d.; da Fonseca-Martins, Alessandra M.; Carneiro, Monique P.D.; Ramos, Tadeu D.; Firmino-Cruz, Luan; Gomes, Daniel C.O.; Rossi-Bergmann, Bartira; de Matos Guedes, Herbert L. 2021. "MPLA and AddaVax® Adjuvants Fail to Promote Intramuscular LaAg Vaccine Protectiveness against Experimental Cutaneous Leishmaniasis" Microorganisms 9, no. 6: 1272. https://doi.org/10.3390/microorganisms9061272

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop