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Article

Multi Locus Sequence Typing and spa Typing of Staphylococcus aureus Isolated from the Milk of Cows with Subclinical Mastitis in Croatia

1
Department of Bacteriology and Parasitology, Croatian Veterinary Institute, 10000 Zagreb, Croatia
2
Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine University of Zagreb, 10000 Zagreb, Croatia
3
Department of Microbiology and Infectious Diseases, with Clinic, Faculty of Veterinary Medicine University of Zagreb, 10000 Zagreb, Croatia
4
Veterinary Laboratory Križevci, Croatian Veterinary Institute, 10000 Zagreb, Croatia
5
Mount Trade Company, 43280 Garešnica, Croatia
*
Author to whom correspondence should be addressed.
Academic Editor: David Rodríguez-Lázaro
Microorganisms 2021, 9(4), 725; https://doi.org/10.3390/microorganisms9040725
Received: 9 March 2021 / Revised: 25 March 2021 / Accepted: 29 March 2021 / Published: 31 March 2021
(This article belongs to the Section Food Microbiology)
Background: The bacterial species S. aureus is the most common causative agent of mastitis in cows in most countries with a dairy industry. The prevalence of infection caused by S. aureus ranges from 2% to more than 50%, and it causes 10–12% of all cases of clinical mastitis. Aim: The objective was to analyze 237 strains of S. aureus isolated from the milk of cows with subclinical mastitis regarding the spa, mecA, mecC and pvl genes and to perform spa and multi-locus sequence typing (MLST). Methods: Sequencing amplified gene sequences was conducted at Macrogen Europe. Ridom StaphType and BioNumerics software was used to analyze obtained sequences of spa and seven housekeeping genes. Results: The spa fragment was present in 204 (86.1%) of strains, while mecA and mecC gene were detected in 10 strains, and the pvl gene was not detected. Spa typing successfully analyzed 153 tested isolates (64.3%), confirming 53 spa types, four of which were new types. The most frequent spa type was t2678 (14%). MLST typed 198 (83.5%) tested strains and defined 32 different allele profiles, of which three were new. The most frequent allele profile was ST133 (20.7%). Six groups (G) and 15 singletons were defined. Conclusion: Taking the number of confirmed spa types and sequence types (STs) into account, it can be concluded that the strains of S. aureus isolated from the milk of cows with subclinical mastitis form a heterogenous group. To check the possible zoonotic potential of isolates it would be necessary to test the persons and other livestock on the farms. View Full-Text
Keywords: S. aureus; mastitis; MLST; spa typing S. aureus; mastitis; MLST; spa typing
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MDPI and ACS Style

Cvetnić, L.; Samardžija, M.; Duvnjak, S.; Habrun, B.; Cvetnić, M.; Jaki Tkalec, V.; Đuričić, D.; Benić, M. Multi Locus Sequence Typing and spa Typing of Staphylococcus aureus Isolated from the Milk of Cows with Subclinical Mastitis in Croatia. Microorganisms 2021, 9, 725. https://doi.org/10.3390/microorganisms9040725

AMA Style

Cvetnić L, Samardžija M, Duvnjak S, Habrun B, Cvetnić M, Jaki Tkalec V, Đuričić D, Benić M. Multi Locus Sequence Typing and spa Typing of Staphylococcus aureus Isolated from the Milk of Cows with Subclinical Mastitis in Croatia. Microorganisms. 2021; 9(4):725. https://doi.org/10.3390/microorganisms9040725

Chicago/Turabian Style

Cvetnić, Luka, Marko Samardžija, Sanja Duvnjak, Boris Habrun, Marija Cvetnić, Vesna Jaki Tkalec, Dražen Đuričić, and Miroslav Benić. 2021. "Multi Locus Sequence Typing and spa Typing of Staphylococcus aureus Isolated from the Milk of Cows with Subclinical Mastitis in Croatia" Microorganisms 9, no. 4: 725. https://doi.org/10.3390/microorganisms9040725

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