Next Article in Journal
Wild Boars Carry Extended-Spectrum β-Lactamase- and AmpC-Producing Escherichia coli
Next Article in Special Issue
The Role of Streptococcus spp. in Bovine Mastitis
Previous Article in Journal
Changes of the Proteome and Acetylome during Transition into the Stationary Phase in the Organohalide-Respiring Dehalococcoides mccartyi Strain CBDB1
Previous Article in Special Issue
Streptococcal Infections in Marine Mammals
Article

Streptococcus suis Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue

1
Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany
2
Institute for Pathology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany
3
Veterinary Centre for Resistance Research, Department of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Hirokazu Kimura
Microorganisms 2021, 9(2), 366; https://doi.org/10.3390/microorganisms9020366
Received: 23 January 2021 / Revised: 6 February 2021 / Accepted: 9 February 2021 / Published: 12 February 2021
(This article belongs to the Special Issue Epidemiology and Pathogenicity of Animal-Adapted Streptococci)
Streptococcus suis is a common pathogen colonising the respiratory tract of pigs. It can cause meningitis, sepsis and pneumonia leading to economic losses in the pig industry worldwide. Cyclooxygenase-2 (COX-2) and its metabolites play an important regulatory role in different biological processes like inflammation modulation and immune activation. In this report we analysed the induction of COX-2 and the production of its metabolite prostaglandin E2 (PGE2) in a porcine precision-cut lung slice (PCLS) model. Using Western blot analysis, we found a time-dependent induction of COX-2 in the infected tissue resulting in increased PGE2 levels. Immunohistological analysis revealed a strong COX-2 expression in the proximity of the bronchioles between the ciliated epithelial cells and the adjacent alveolar tissue. The morphology, location and vimentin staining suggested that these cells are subepithelial bronchial fibroblasts. Furthermore, we showed that COX-2 expression as well as PGE2 production was detected following infection with two prevalent S. suis serotypes and that the pore-forming toxin suilysin played an important role in this process. Therefore, this study provides new insights in the response of porcine lung cells to S. suis infections and serves as a basis for further studies to define the role of COX-2 and its metabolites in the inflammatory response in porcine lung tissue during infections with S. suis. View Full-Text
Keywords: Streptococcus suis; COX-2; cyclooxygenase; inflammation; lung infection; precision-cut lung slices Streptococcus suis; COX-2; cyclooxygenase; inflammation; lung infection; precision-cut lung slices
Show Figures

Figure 1

MDPI and ACS Style

Dresen, M.; Schenk, J.; Berhanu Weldearegay, Y.; Vötsch, D.; Baumgärtner, W.; Valentin-Weigand, P.; Nerlich, A. Streptococcus suis Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue. Microorganisms 2021, 9, 366. https://doi.org/10.3390/microorganisms9020366

AMA Style

Dresen M, Schenk J, Berhanu Weldearegay Y, Vötsch D, Baumgärtner W, Valentin-Weigand P, Nerlich A. Streptococcus suis Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue. Microorganisms. 2021; 9(2):366. https://doi.org/10.3390/microorganisms9020366

Chicago/Turabian Style

Dresen, Muriel, Josephine Schenk, Yenehiwot Berhanu Weldearegay, Désirée Vötsch, Wolfgang Baumgärtner, Peter Valentin-Weigand, and Andreas Nerlich. 2021. "Streptococcus suis Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue" Microorganisms 9, no. 2: 366. https://doi.org/10.3390/microorganisms9020366

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop