Independence of a Marine Unicellular Diazotroph to the Presence of NO₃⁻

Marine nitrogen (N₂) fixation was historically considered to be absent or reduced in nitrate (NO₃⁻) rich environments. This is commonly attributed to the lower energetic cost of NO₃⁻ uptake compared to diazotrophy in oxic environments. This paradigm often contributes to making inferences about diazotroph distribution and activity in the ocean, and is also often used in biogeochemical ocean models. To assess the general validity of this paradigm beyond the traditionally used model organism Trichodesmium spp., we grew cultures of the unicellular cyanobacterium Crocosphaera watsonii WH8501 long term in medium containing replete concentrations of NO₃⁻. NO₃⁻ uptake was measured in comparison to N₂ fixation to assess the cultures’ nitrogen source preferences. We further measured culture growth rate, cell stoichiometry, and carbon fixation rate to determine if the presence of NO₃⁻ had any effect on cell metabolism. We found that uptake of NO₃⁻ by this strain of Crocosphaera was minimal in comparison to other N sources (~2–4% of total uptake). Furthermore, availability of NO₃⁻ did not statistically alter N₂ fixation rate nor any aspect of cell physiology or metabolism measured (cellular growth rate, cell stoichiometry, cell size, nitrogen fixation rate, nitrogenase activity) in comparison to a NO₃⁻ free control culture. These results demonstrate the capability of a marine diazotroph to fix nitrogen and grow independently of NO₃⁻. This lack of sensitivity of diazotrophy to NO₃⁻ suggests that assumptions often made about, and model formulations of, N₂ fixation should be reconsidered.