Next Article in Journal
Enteroviral Pathogenesis of Type 1 Diabetes: The Role of Natural Killer Cells
Next Article in Special Issue
Ongoing Coevolution of Wolbachia and a Widespread Invasive Ant, Anoplolepis gracilipes
Previous Article in Journal
Malaria Elimination in Costa Rica: Changes in Treatment and Mass Drug Administration
Previous Article in Special Issue
Evidence for Common Horizontal Transmission of Wolbachia among Ants and Ant Crickets: Kleptoparasitism Added to the List
Article

Isolation and Propagation of Laboratory Strains and a Novel Flea-Derived Field Strain of Wolbachia in Tick Cell Lines

1
Tropical Infectious Diseases Research and Education Centre, Level 2, High Impact Research Building, University of Malaya, Kuala Lumpur 50603, Malaysia
2
Department of Entomology, University of Minnesota, 219 Hodson Hall, 1980 Folwell Avenue, Saint Paul, MN 55108, USA
3
Department of Infection Biology and Microbiome, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, 146 Brownlow Hill, Liverpool L3 5RF, UK
4
College of Veterinary Medicine, University of Al-Qadisiyah, Qadisiyah 54004, Iraq
5
Departments of Vector Biology and Tropical Disease Biology, Centre for Neglected Tropical Disease, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(7), 988; https://doi.org/10.3390/microorganisms8070988
Received: 10 June 2020 / Revised: 29 June 2020 / Accepted: 30 June 2020 / Published: 1 July 2020
(This article belongs to the Special Issue Wolbachia and Other Selfish Symbionts of Arthropods)
Wolbachia are intracellular endosymbionts of several invertebrate taxa, including insects and nematodes. Although Wolbachia DNA has been detected in ticks, its presence is generally associated with parasitism by insects. To determine whether or not Wolbachia can infect and grow in tick cells, cell lines from three tick species, Ixodes scapularis, Ixodes ricinus and Rhipicephalus microplus, were inoculated with Wolbachia strains wStri and wAlbB isolated from mosquito cell lines. Homogenates prepared from fleas collected from cats in Malaysia were inoculated into an I. scapularis cell line. Bacterial growth and identity were monitored by microscopy and PCR amplification and sequencing of fragments of Wolbachia genes. The wStri strain infected Ixodes spp. cells and was maintained through 29 passages. The wAlbB strain successfully infected Ixodes spp. and R. microplus cells and was maintained through 2–5 passages. A novel strain of Wolbachia belonging to the supergroup F, designated wCfeF, was isolated in I. scapularis cells from a pool of Ctenocephalides sp. cat fleas and maintained in vitro through two passages over nine months. This is the first confirmed isolation of a Wolbachia strain from a flea and the first isolation of any Wolbachia strain outside the “pandemic” A and B supergroups. The study demonstrates that tick cells can host multiple Wolbachia strains, and can be added to panels of insect cell lines to improve success rates in isolation of field strains of Wolbachia. View Full-Text
Keywords: Wolbachia; tick cell line; Ctenocephalides; flea; Malaysia; in vitro culture; phylogeny Wolbachia; tick cell line; Ctenocephalides; flea; Malaysia; in vitro culture; phylogeny
Show Figures

Figure 1

MDPI and ACS Style

Khoo, J.J.; Kurtti, T.J.; Husin, N.A.; Beliavskaia, A.; Lim, F.S.; Zulkifli, M.M.S.; Al-Khafaji, A.M.; Hartley, C.; Darby, A.C.; Hughes, G.L.; AbuBakar, S.; Makepeace, B.L.; Bell-Sakyi, L. Isolation and Propagation of Laboratory Strains and a Novel Flea-Derived Field Strain of Wolbachia in Tick Cell Lines. Microorganisms 2020, 8, 988. https://doi.org/10.3390/microorganisms8070988

AMA Style

Khoo JJ, Kurtti TJ, Husin NA, Beliavskaia A, Lim FS, Zulkifli MMS, Al-Khafaji AM, Hartley C, Darby AC, Hughes GL, AbuBakar S, Makepeace BL, Bell-Sakyi L. Isolation and Propagation of Laboratory Strains and a Novel Flea-Derived Field Strain of Wolbachia in Tick Cell Lines. Microorganisms. 2020; 8(7):988. https://doi.org/10.3390/microorganisms8070988

Chicago/Turabian Style

Khoo, Jing J.; Kurtti, Timothy J.; Husin, Nurul A.; Beliavskaia, Alexandra; Lim, Fang S.; Zulkifli, Mulya M.S.; Al-Khafaji, Alaa M.; Hartley, Catherine; Darby, Alistair C.; Hughes, Grant L.; AbuBakar, Sazaly; Makepeace, Benjamin L.; Bell-Sakyi, Lesley. 2020. "Isolation and Propagation of Laboratory Strains and a Novel Flea-Derived Field Strain of Wolbachia in Tick Cell Lines" Microorganisms 8, no. 7: 988. https://doi.org/10.3390/microorganisms8070988

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop