Next Article in Journal
Biological Control of Verticillium Wilt on Olive Trees by the Salt-Tolerant Strain Bacillus velezensis XT1
Next Article in Special Issue
Low-Temperature Biosurfactants from Polar Microbes
Previous Article in Journal
Cytomegalovirus and Inflammatory Bowel Diseases (IBD) with a Special Focus on the Link with Ulcerative Colitis (UC)
Previous Article in Special Issue
In Vitro and Ex Vivo Antibiofilm Activity of a Lipopeptide Biosurfactant Produced by the Entomopathogenic Beauveria bassiana Strain against Microsporum canis
Article

Biosynthesis and Antimicrobial Activity of Pseudodesmin and Viscosinamide Cyclic Lipopeptides Produced by Pseudomonads Associated with the Cocoyam Rhizosphere

1
Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium
2
Unit for Environmental Sciences and Management, Faculty of Natural and Agricultural Sciences, North-West University, 2520 Potchefstroom, South Africa
3
NMR and Structure Analysis Unit, Department of Organic and Macromolecular Chemistry, Faculty of Science, Ghent University, Krijgslaan 281, B-9000 Gent, Belgium
4
Jay PJ Biotechnology Laboratory, Institute of Agricultural Research for Development (IRAD), Ekona, P. M. B 25 Buea, Cameroon
5
Department of Microbiology, Obafemi Awolowo University, 220005 Ile-Ife, Osun State, Nigeria
6
Plant Pathology Unit, National Root Crops Research Institute (NRCRI), 440001 Umudike, Abia State, Nigeria
7
Faculty of Agriculture, Department of Crop, Horticulture and Landscape Design, Ekiti State University (EKSU), 360211 Ado-Ekiti, Nigeria
8
Centre of Microbial and Plant Genetics, Faculty of Bioscience Engineering, KU Leuven, 3001 Heverlee, Belgium
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(7), 1079; https://doi.org/10.3390/microorganisms8071079
Received: 14 June 2020 / Revised: 13 July 2020 / Accepted: 15 July 2020 / Published: 20 July 2020
(This article belongs to the Special Issue Microbial Biosurfactants)
Pseudomonas cyclic lipopeptides (CLPs) are encoded non-ribosomally by biosynthetic gene clusters (BGCs) and possess diverse biological activities. In this study, we conducted chemical structure and BGC analyses with antimicrobial activity assays for two CLPs produced by Pseudomonas strains isolated from the cocoyam rhizosphere in Cameroon and Nigeria. LC-MS and NMR analyses showed that the Pseudomonas sp. COR52 and A2W4.9 produce pseudodesmin and viscosinamide, respectively. These CLPs belong to the Viscosin group characterized by a nonapeptidic moiety with a 7-membered macrocycle. Similar to other Viscosin-group CLPs, the initiatory non-ribosomal peptide synthetase (NRPS) gene of the viscosinamide BGC is situated remotely from the other two NRPS genes. In contrast, the pseudodesmin genes are all clustered in a single genomic locus. Nano- to micromolar levels of pseudodesmin and viscosinamide led to the hyphal distortion and/or disintegration of Rhizoctonia solani AG2-2 and Pythium myriotylum CMR1, whereas similar levels of White Line-Inducing Principle (WLIP), another member of the Viscosin group, resulted in complete lysis of both soil-borne phytopathogens. In addition to the identification of the biosynthetic genes of these two CLPs and the demonstration of their interaction with soil-borne pathogens, this study provides further insights regarding evolutionary divergence within the Viscosin group. View Full-Text
Keywords: viscosin group; WLIP; evolution; CLP perception; defense response; Pythium myriotylum; Rhizoctonia solani; swarming motility viscosin group; WLIP; evolution; CLP perception; defense response; Pythium myriotylum; Rhizoctonia solani; swarming motility
Show Figures

Figure 1

MDPI and ACS Style

Oni, F.E.; Geudens, N.; Adiobo, A.; Omoboye, O.O.; Enow, E.A.; Onyeka, J.T.; Salami, A.E.; De Mot, R.; Martins, J.C.; Höfte, M. Biosynthesis and Antimicrobial Activity of Pseudodesmin and Viscosinamide Cyclic Lipopeptides Produced by Pseudomonads Associated with the Cocoyam Rhizosphere. Microorganisms 2020, 8, 1079. https://doi.org/10.3390/microorganisms8071079

AMA Style

Oni FE, Geudens N, Adiobo A, Omoboye OO, Enow EA, Onyeka JT, Salami AE, De Mot R, Martins JC, Höfte M. Biosynthesis and Antimicrobial Activity of Pseudodesmin and Viscosinamide Cyclic Lipopeptides Produced by Pseudomonads Associated with the Cocoyam Rhizosphere. Microorganisms. 2020; 8(7):1079. https://doi.org/10.3390/microorganisms8071079

Chicago/Turabian Style

Oni, Feyisara E., Niels Geudens, Amayana Adiobo, Olumide O. Omoboye, Elsie A. Enow, Joseph T. Onyeka, Ayodeji E. Salami, René De Mot, José C. Martins, and Monica Höfte. 2020. "Biosynthesis and Antimicrobial Activity of Pseudodesmin and Viscosinamide Cyclic Lipopeptides Produced by Pseudomonads Associated with the Cocoyam Rhizosphere" Microorganisms 8, no. 7: 1079. https://doi.org/10.3390/microorganisms8071079

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop