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Article

Unraveling the Microbiota of Natural Black cv. Kalamata Fermented Olives through 16S and ITS Metataxonomic Analysis

1
Laboratory of Dairy Research, Department of Food Science and Human Nutrition, School of Food and Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece
2
Laboratory of Food Microbiology and Biotechnology, Department of Food Science and Human Nutrition, School of Food and Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece
*
Authors to whom correspondence should be addressed.
Microorganisms 2020, 8(5), 672; https://doi.org/10.3390/microorganisms8050672
Received: 17 April 2020 / Revised: 2 May 2020 / Accepted: 4 May 2020 / Published: 6 May 2020
(This article belongs to the Special Issue Food Microbial Diversity)
Kalamata natural black olives are one of the most economically important Greek varieties. The microbial ecology of table olives is highly influenced by the co-existence of bacteria and yeasts/fungi, as well as the physicochemical parameters throughout the fermentation. Therefore, the aim of this study was the identification of bacterial and yeast/fungal microbiota of both olives and brines obtained from 29 cv. Kalamata olive samples industrially fermented in the two main producing geographical regions of Greece, namely Aitoloakarnania and Messinia/Lakonia. The potential microbial biogeography association between certain taxa and geographical area was also assessed. The dominant bacterial family identified in olive and brine samples from both regions was Lactobacillaceae, presenting, however, higher average abundances in the samples from Aitoloakarnania compared to Messinia/Lakonia. At the genus level, Lactobacillus, Celerinatantimonas, Propionibacterium and Pseudomonas were the most abundant. In addition, the yeasts/fungal communities were less diverse compared to those of bacteria, with Pichiaceae being the dominant family and Pichia, Ogataea, and Saccharomyces being the most abundant genera. To the best of our knowledge, this is the first report on the microbiota of both olives and brines of cv. Kalamata black olives fermented on an industrial scale between two geographical regions of Greece using metagenomics analysis. View Full-Text
Keywords: table olives; Kalamata olives; Greek style fermentation; microbiological analysis; 16S metagenomics analysis; ITS metagenomics analysis; microbiota; high-throughput sequencing table olives; Kalamata olives; Greek style fermentation; microbiological analysis; 16S metagenomics analysis; ITS metagenomics analysis; microbiota; high-throughput sequencing
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MDPI and ACS Style

Kazou, M.; Tzamourani, A.; Panagou, E.Z.; Tsakalidou, E. Unraveling the Microbiota of Natural Black cv. Kalamata Fermented Olives through 16S and ITS Metataxonomic Analysis. Microorganisms 2020, 8, 672. https://doi.org/10.3390/microorganisms8050672

AMA Style

Kazou M, Tzamourani A, Panagou EZ, Tsakalidou E. Unraveling the Microbiota of Natural Black cv. Kalamata Fermented Olives through 16S and ITS Metataxonomic Analysis. Microorganisms. 2020; 8(5):672. https://doi.org/10.3390/microorganisms8050672

Chicago/Turabian Style

Kazou, Maria, Aikaterini Tzamourani, Efstathios Z. Panagou, and Effie Tsakalidou. 2020. "Unraveling the Microbiota of Natural Black cv. Kalamata Fermented Olives through 16S and ITS Metataxonomic Analysis" Microorganisms 8, no. 5: 672. https://doi.org/10.3390/microorganisms8050672

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